微藻基细菌通过增强肠道定植和体内平衡治疗ASD。

IF 12.4 1区 医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
Theranostics Pub Date : 2025-01-13 eCollection Date: 2025-01-01 DOI:10.7150/thno.103737
Rongrong Yang, Li Ma, Huan Peng, Yifang Zhai, Gengyao Zhou, Linjuan Zhang, Lixia Zhuo, Wei Wu, Yuxi Guo, Jiao Han, Linlin Jing, Xinyu Zhou, Xiancang Ma, Yan Li
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引用次数: 0

摘要

理论基础:外源性补充有益肠道细菌可以通过肠脑相互作用减轻精神障碍(如自闭症谱系障碍(ASD))的行为症状。然而,肠道定植有限阻碍了诸如罗伊氏乳杆菌(L. reuteri)等有益菌治疗ASD的应用。方法:利用螺旋藻(Spirulina platensis, SP)作为肠道细菌的天然微载体,采用更安全、更天然的结合方式,将肠道细菌与SP表面结合,产生SP肠道细菌。由于SP在肠道内的黏附效率高,停留时间长,SP-肠细菌具有更强的肠道定植能力,对ASD的治疗效果更好。结果:SP是一种有效的载体,可以结合和递送不同形状、不同革兰氏染色性质的细菌。sp -肠道细菌在体外和体内均表现出增强的定植能力。进一步研究表明,SP-L。罗伊氏乳杆菌的肠道定植效率高于罗伊氏乳杆菌。sp l。罗伊菌通过调节肠道菌群、增强肠道屏障完整性、减少脂多糖进入血液和介导室旁丘脑核的神经炎症反应,在ASD小鼠模型中显示出更强的治疗作用。结论:本研究报道了一种微藻辅助肠道细菌递送系统,可增强肠-脑轴或其他肠道相关疾病的肠道细菌移植。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microalgae-based bacteria for oral treatment of ASD through enhanced intestinal colonization and homeostasis.

Rationale: Exogenous supplementation of beneficial intestinal bacteria can alleviate the behavioural symptoms of psychiatric disorders, such as autism spectrum disorder (ASD), through gut-brain interactions. However, the application of beneficial bacteria, such as Lactobacillus reuteri (L. reuteri), for treating ASD is hindered by limited gut colonization. Methods: Utilizing Spirulina platensis (SP) as a natural microcarrier for intestinal bacteria, a safer and more natural binding approach was employed to bind intestinal bacteria to the surface of SP to produce SP-intestinal bacteria. Due to the high adhesion efficiency and long residence time of SP in the intestines, SP-intestinal bacteria exhibit stronger intestinal colonization ability and better therapeutic effects on ASD. Results: SP is an effective carrier that can bind and deliver bacteria of different shapes and with different gram-staining properties. SP-intestinal bacteria exhibited enhanced colonization capabilities both ex vivo and in vivo. Further research showed that SP-L. reuteri had greater intestinal colonization efficiency than L. reuteri. SP-L. reuteri showed a stronger therapeutic effect on alleviating social deficits in an ASD mouse model by modulating the gut microbiota, enhancing intestinal barrier integrity, reducing lipopolysaccharide entry into the blood and mediating the neuroinflammatory response in the paraventricular thalamic nucleus. Conclusions: This study reports a microalgae-assisted intestinal bacterial delivery system for enhancing intestinal bacterial transplantation for gut-brain axis- or other intestinal-related diseases.

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来源期刊
Theranostics
Theranostics MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
25.40
自引率
1.60%
发文量
433
审稿时长
1 months
期刊介绍: Theranostics serves as a pivotal platform for the exchange of clinical and scientific insights within the diagnostic and therapeutic molecular and nanomedicine community, along with allied professions engaged in integrating molecular imaging and therapy. As a multidisciplinary journal, Theranostics showcases innovative research articles spanning fields such as in vitro diagnostics and prognostics, in vivo molecular imaging, molecular therapeutics, image-guided therapy, biosensor technology, nanobiosensors, bioelectronics, system biology, translational medicine, point-of-care applications, and personalized medicine. Encouraging a broad spectrum of biomedical research with potential theranostic applications, the journal rigorously peer-reviews primary research, alongside publishing reviews, news, and commentary that aim to bridge the gap between the laboratory, clinic, and biotechnology industries.
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