Matteo Vatta, Ellen Regalado, Michael Parfenov, Dan Swartzlander, Andrea Nagl, Meghan Mannello, Rachel Lewis, Daniel Clemens, John Garcia, Rachel E Ellsworth, Ana Morales, Yi-Lee Ting, Swaroop Aradhya
{"title":"bbbb74000例TTN截断变异分析揭示了新的临床相关基因区域","authors":"Matteo Vatta, Ellen Regalado, Michael Parfenov, Dan Swartzlander, Andrea Nagl, Meghan Mannello, Rachel Lewis, Daniel Clemens, John Garcia, Rachel E Ellsworth, Ana Morales, Yi-Lee Ting, Swaroop Aradhya","doi":"10.1161/CIRCGEN.124.004982","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Truncating variants (TTNtvs) in the titin (<i>TTN</i>) gene have been associated with cardiomyopathies or arrhythmias (C/A) and autosomal recessive neuromuscular diseases (NM). However, the clinical significance of TTNtvs across the entire coding sequence of <i>TTN</i> has not been comprehensively assessed. The purpose of this study was to examine the burden of TTNtvs in C/A and NM cases compared with controls in the genome aggregation database.</p><p><strong>Methods: </strong>This was a retrospective study of probands who underwent multigene testing (49 740 C/A panel, 24 514 NM panel) that included <i>TTN</i> from November 2017 to October 2021. Burden testing was performed using controls in the genome aggregation database v3.1.2 database, and the analysis was stratified by exon/band location and exon usage in cardiac or skeletal muscle. Frequency and odds ratio of TTNtv alleles in C/A or NM cases and genome aggregation database controls were measured.</p><p><strong>Results: </strong>There were 2446 (4.9%) C/A and 482 (2.0%) NM cases with 2446 and 528 TTNtv alleles, respectively. TTNtvs in all bands were significantly enriched in both C/A and NM cases compared with controls. A significant enrichment of TTNtvs in C/A was observed for exon 358 of the M-band (odds ratio, 2.55 [95% CI, 1.85-3.54]) but not the other M-band exons.</p><p><strong>Conclusions: </strong>In the largest single-site cohort of C/A and NM cases with TTNtvs, an enrichment of TTNtvs across <i>TTN</i> was observed. These findings expand the clinically relevant regions of <i>TTN</i>.</p>","PeriodicalId":10326,"journal":{"name":"Circulation: Genomic and Precision Medicine","volume":" ","pages":"e004982"},"PeriodicalIF":6.0000,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Analysis of <i>TTN</i> Truncating Variants in >74 000 Cases Reveals New Clinically Relevant Gene Regions.\",\"authors\":\"Matteo Vatta, Ellen Regalado, Michael Parfenov, Dan Swartzlander, Andrea Nagl, Meghan Mannello, Rachel Lewis, Daniel Clemens, John Garcia, Rachel E Ellsworth, Ana Morales, Yi-Lee Ting, Swaroop Aradhya\",\"doi\":\"10.1161/CIRCGEN.124.004982\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Truncating variants (TTNtvs) in the titin (<i>TTN</i>) gene have been associated with cardiomyopathies or arrhythmias (C/A) and autosomal recessive neuromuscular diseases (NM). However, the clinical significance of TTNtvs across the entire coding sequence of <i>TTN</i> has not been comprehensively assessed. The purpose of this study was to examine the burden of TTNtvs in C/A and NM cases compared with controls in the genome aggregation database.</p><p><strong>Methods: </strong>This was a retrospective study of probands who underwent multigene testing (49 740 C/A panel, 24 514 NM panel) that included <i>TTN</i> from November 2017 to October 2021. Burden testing was performed using controls in the genome aggregation database v3.1.2 database, and the analysis was stratified by exon/band location and exon usage in cardiac or skeletal muscle. Frequency and odds ratio of TTNtv alleles in C/A or NM cases and genome aggregation database controls were measured.</p><p><strong>Results: </strong>There were 2446 (4.9%) C/A and 482 (2.0%) NM cases with 2446 and 528 TTNtv alleles, respectively. TTNtvs in all bands were significantly enriched in both C/A and NM cases compared with controls. A significant enrichment of TTNtvs in C/A was observed for exon 358 of the M-band (odds ratio, 2.55 [95% CI, 1.85-3.54]) but not the other M-band exons.</p><p><strong>Conclusions: </strong>In the largest single-site cohort of C/A and NM cases with TTNtvs, an enrichment of TTNtvs across <i>TTN</i> was observed. These findings expand the clinically relevant regions of <i>TTN</i>.</p>\",\"PeriodicalId\":10326,\"journal\":{\"name\":\"Circulation: Genomic and Precision Medicine\",\"volume\":\" \",\"pages\":\"e004982\"},\"PeriodicalIF\":6.0000,\"publicationDate\":\"2025-02-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Circulation: Genomic and Precision Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1161/CIRCGEN.124.004982\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CARDIAC & CARDIOVASCULAR SYSTEMS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Circulation: Genomic and Precision Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1161/CIRCGEN.124.004982","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CARDIAC & CARDIOVASCULAR SYSTEMS","Score":null,"Total":0}
Analysis of TTN Truncating Variants in >74 000 Cases Reveals New Clinically Relevant Gene Regions.
Background: Truncating variants (TTNtvs) in the titin (TTN) gene have been associated with cardiomyopathies or arrhythmias (C/A) and autosomal recessive neuromuscular diseases (NM). However, the clinical significance of TTNtvs across the entire coding sequence of TTN has not been comprehensively assessed. The purpose of this study was to examine the burden of TTNtvs in C/A and NM cases compared with controls in the genome aggregation database.
Methods: This was a retrospective study of probands who underwent multigene testing (49 740 C/A panel, 24 514 NM panel) that included TTN from November 2017 to October 2021. Burden testing was performed using controls in the genome aggregation database v3.1.2 database, and the analysis was stratified by exon/band location and exon usage in cardiac or skeletal muscle. Frequency and odds ratio of TTNtv alleles in C/A or NM cases and genome aggregation database controls were measured.
Results: There were 2446 (4.9%) C/A and 482 (2.0%) NM cases with 2446 and 528 TTNtv alleles, respectively. TTNtvs in all bands were significantly enriched in both C/A and NM cases compared with controls. A significant enrichment of TTNtvs in C/A was observed for exon 358 of the M-band (odds ratio, 2.55 [95% CI, 1.85-3.54]) but not the other M-band exons.
Conclusions: In the largest single-site cohort of C/A and NM cases with TTNtvs, an enrichment of TTNtvs across TTN was observed. These findings expand the clinically relevant regions of TTN.
期刊介绍：
Circulation: Genomic and Precision Medicine is a distinguished journal dedicated to advancing the frontiers of cardiovascular genomics and precision medicine. It publishes a diverse array of original research articles that delve into the genetic and molecular underpinnings of cardiovascular diseases. The journal's scope is broad, encompassing studies from human subjects to laboratory models, and from in vitro experiments to computational simulations.
Circulation: Genomic and Precision Medicine is committed to publishing studies that have direct relevance to human cardiovascular biology and disease, with the ultimate goal of improving patient care and outcomes. The journal serves as a platform for researchers to share their groundbreaking work, fostering collaboration and innovation in the field of cardiovascular genomics and precision medicine.
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