Natasha Haskey, Clara Letef, James A Sousa, Munazza Yousuf, Lorian M Taylor, Derek M McKay, Christopher Ma, Subrata Ghosh, Deanna L Gibson, Maitreyi Raman
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Despite their significance, research on dietary FA subtypes and their effects on inflammation and oxidative stress in IBD is limited.</p><p><strong>Objective: </strong>We investigated the association between dietary FA intake, the erythrocyte membrane FA composition (EMFA), and inflammation and oxidative stress markers in patients with mild-moderate luminal Crohn's Disease (CD) participating in the CD Therapeutic Dietary Intervention (CD-TDI).</p><p><strong>Design: </strong>A cross-sectional analysis was performed on 24 participants (13 CD-TDI, 11 habitual diet controls) from a 13-week randomized controlled trial assessing the efficacy of CD-TDI in inducing clinical and biomarker remission in CD.</p><p><strong>Methods: </strong>EMFA was analyzed using direct-injection gas chromatography, and dietary FA intake was assessed using the ASA 24-h Dietary Assessment Tool<sup>®</sup>.</p><p><strong>Results: </strong>The CD-TDI group showed a significant increase in dietary n-3 polyunsaturated fatty acids (PUFA) at Week 13 (<i>p</i> = 0.04) compared to no changes in the control group. Participants on the CD-TDI also demonstrated a significant reduction in total fat, saturated fat, and arachidonic acid (AA) (<i>p</i> < 0.01). EMFA analysis revealed lower percentages of AA (<i>p</i> = 0.03) in the CD-TDI group. Positive correlations were observed between C-reactive protein, fecal calprotectin, and dietary stearic acid (<i>p</i> < 0.05). Inverse correlations were found between malondialdehyde (MDA) and the Mediterranean Diet Score (<i>r</i> = -0.67) as well as MDA and the intake of whole fruit, legumes, and nuts/seeds (<i>r</i> > -0.50).</p><p><strong>Conclusion: </strong>The CD-TDI significantly increased dietary n-3 PUFA intake, reduced pro-inflammatory n-6 PUFA (AA), and improved markers of oxidative stress, supporting its potential in CD management. The cell membrane fatty acid profile might be a therapeutic target in CD.</p><p><strong>Trial registration: </strong>NCT04596566.</p>","PeriodicalId":48770,"journal":{"name":"Therapeutic Advances in Gastroenterology","volume":"18 ","pages":"17562848251314827"},"PeriodicalIF":3.9000,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11831646/pdf/","citationCount":"0","resultStr":"{\"title\":\"Exploring the connection between erythrocyte membrane fatty acid composition and oxidative stress in patients undergoing the Crohn's disease Therapeutic Diet Intervention (CD-TDI).\",\"authors\":\"Natasha Haskey, Clara Letef, James A Sousa, Munazza Yousuf, Lorian M Taylor, Derek M McKay, Christopher Ma, Subrata Ghosh, Deanna L Gibson, Maitreyi Raman\",\"doi\":\"10.1177/17562848251314827\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Dietary fatty acids (FA) are crucial to the pathophysiology of inflammatory bowel disease (IBD), influencing systemic and gut inflammatory responses. 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引用次数: 0
摘要
背景：膳食脂肪酸（FA）对炎症性肠病（IBD）的病理生理至关重要，影响全身和肠道炎症反应。膳食FA摄入量影响重要细胞膜的脂肪酸谱，这可能是炎症介质的来源。尽管它们具有重要意义，但关于膳食FA亚型及其对IBD炎症和氧化应激影响的研究有限。目的：研究参加CD治疗性饮食干预（CD- tdi）的轻、中度luminal Crohn's Disease （CD）患者膳食FA摄入量、红细胞膜FA组成（EMFA）以及炎症和氧化应激标志物之间的关系。设计：对来自13周随机对照试验的24名参与者（13名CD-TDI， 11名习惯饮食对照）进行横断分析，以评估CD-TDI诱导cd临床和生物标志物缓解的疗效。方法：采用直接注射气相色谱法分析EMFA，使用ASA 24 h膳食评估工具®评估膳食FA摄入量。结果：与对照组相比，CD-TDI组在第13周的膳食n-3多不饱和脂肪酸（PUFA）显著增加（p = 0.04）。CD-TDI组的参与者也显示出总脂肪、饱和脂肪和花生四烯酸（AA）的显著减少（p p = 0.03）。c反应蛋白、粪便钙保护蛋白与膳食硬脂酸呈正相关（p r = -0.67），丙二醛与整个水果、豆类和坚果/种子的摄入量呈正相关（p r = -0.50）。结论：CD- tdi显著增加了饮食中n-3 PUFA的摄入量，降低了促炎n-6 PUFA (AA)，改善了氧化应激标志物，支持其在CD治疗中的潜力。细胞膜脂肪酸谱可能成为cd的治疗靶点。试验注册号：NCT04596566。
Exploring the connection between erythrocyte membrane fatty acid composition and oxidative stress in patients undergoing the Crohn's disease Therapeutic Diet Intervention (CD-TDI).
Background: Dietary fatty acids (FA) are crucial to the pathophysiology of inflammatory bowel disease (IBD), influencing systemic and gut inflammatory responses. Dietary FA intake influences the fatty acid profiles of vital cell membranes, which might be a source of inflammatory mediators. Despite their significance, research on dietary FA subtypes and their effects on inflammation and oxidative stress in IBD is limited.
Objective: We investigated the association between dietary FA intake, the erythrocyte membrane FA composition (EMFA), and inflammation and oxidative stress markers in patients with mild-moderate luminal Crohn's Disease (CD) participating in the CD Therapeutic Dietary Intervention (CD-TDI).
Design: A cross-sectional analysis was performed on 24 participants (13 CD-TDI, 11 habitual diet controls) from a 13-week randomized controlled trial assessing the efficacy of CD-TDI in inducing clinical and biomarker remission in CD.
Methods: EMFA was analyzed using direct-injection gas chromatography, and dietary FA intake was assessed using the ASA 24-h Dietary Assessment Tool®.
Results: The CD-TDI group showed a significant increase in dietary n-3 polyunsaturated fatty acids (PUFA) at Week 13 (p = 0.04) compared to no changes in the control group. Participants on the CD-TDI also demonstrated a significant reduction in total fat, saturated fat, and arachidonic acid (AA) (p < 0.01). EMFA analysis revealed lower percentages of AA (p = 0.03) in the CD-TDI group. Positive correlations were observed between C-reactive protein, fecal calprotectin, and dietary stearic acid (p < 0.05). Inverse correlations were found between malondialdehyde (MDA) and the Mediterranean Diet Score (r = -0.67) as well as MDA and the intake of whole fruit, legumes, and nuts/seeds (r > -0.50).
Conclusion: The CD-TDI significantly increased dietary n-3 PUFA intake, reduced pro-inflammatory n-6 PUFA (AA), and improved markers of oxidative stress, supporting its potential in CD management. The cell membrane fatty acid profile might be a therapeutic target in CD.
期刊介绍：
Therapeutic Advances in Gastroenterology is an open access journal which delivers the highest quality peer-reviewed original research articles, reviews, and scholarly comment on pioneering efforts and innovative studies in the medical treatment of gastrointestinal and hepatic disorders. The journal has a strong clinical and pharmacological focus and is aimed at an international audience of clinicians and researchers in gastroenterology and related disciplines, providing an online forum for rapid dissemination of recent research and perspectives in this area.
The editors welcome original research articles across all areas of gastroenterology and hepatology.
The journal publishes original research articles and review articles primarily. Original research manuscripts may include laboratory, animal or human/clinical studies – all phases. Letters to the Editor and Case Reports will also be considered.
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