高聚合度壳寡糖的酶法生产及其在酱油保鲜中的潜在应用

IF 3.4 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Enzyme and Microbial Technology Pub Date : 2025-02-17 DOI:10.1016/j.enzmictec.2025.110608

Yihao Liu , Guangru Sun , Yimeng Lou , Peng Cheng , Qian Song , Wen Lv , Chunling Wang

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Enzymatic production of chitooligosaccharides with high degree of polymerisations and their potential application to soy sauce preservation

Chitooligosaccharides (COSs) with a high degree of polymerisation (DP 5–10) have been reported to possess diverse bioactivities. Thus, the development of novel methods for the acquisition of high-DP COSs has become increasingly significant. In the study, a novel GH family 46 chitosanase gene (ThCsn46) was expressed and characterized. ThCsn46 was further applied to COSs production, and the highest yield of 95.7 % (143.6 g/L) was obtained using 15 % (w/v) of chitosan as the substrate. The proportion of high-DP COSs occupied 40.6 % of the total COSs. Moreover, the high (GlcN)₆ content was achieved. The total viable count (TVC) and amino acid nitrogen (AAN) of soy sauce incorporated with 0.1 % (w/v) of COSs were better than that of the negative control. The potential of ThCsn46 for application in the production of COSs and the preservation of soy sauce is significant. The green and efficient bioproduction process represents a promising way for further research.

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来源期刊

Enzyme and Microbial Technology 生物-生物工程与应用微生物

CiteScore

7.60

自引率

5.90%

发文量

142

审稿时长

38 days

期刊介绍： Enzyme and Microbial Technology is an international, peer-reviewed journal publishing original research and reviews, of biotechnological significance and novelty, on basic and applied aspects of the science and technology of processes involving the use of enzymes, micro-organisms, animal cells and plant cells. We especially encourage submissions on: Biocatalysis and the use of Directed Evolution in Synthetic Biology and Biotechnology Biotechnological Production of New Bioactive Molecules, Biomaterials, Biopharmaceuticals, and Biofuels New Imaging Techniques and Biosensors, especially as applicable to Healthcare and Systems Biology New Biotechnological Approaches in Genomics, Proteomics and Metabolomics Metabolic Engineering, Biomolecular Engineering and Nanobiotechnology Manuscripts which report isolation, purification, immobilization or utilization of organisms or enzymes which are already well-described in the literature are not suitable for publication in EMT, unless their primary purpose is to report significant new findings or approaches which are of broad biotechnological importance. Similarly, manuscripts which report optimization studies on well-established processes are inappropriate. EMT does not accept papers dealing with mathematical modeling unless they report significant, new experimental data.