辣椒（Capsicum annuum L.）抗卷曲叶病毒相关SSR标记的分子定位与开发

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2025-02-12 DOI:10.1016/j.ygeno.2025.111015

K. Sai Timmarao , Naresh Ponnam , D.C. Lakshmanareddy , M. Krishna Reddy , V. Venkataravanappa , P. Roshini , Mahebub Shaik , B.P. Manoj , K. Madhavi Reddy

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引用次数: 0

摘要

辣椒卷曲叶病毒（ChLCV）是由白蝇传播的begomovirus引起的一种辣椒卷曲叶病毒，对全世界辣椒的种植和生产构成严重威胁。探索寄主植物的抗性及其相关分子标记的鉴定将加速抗性育种。对IHR4615(R) × IHR2451(S) F2群体进行QTL-seq分析，确定与ChLCV-Raichur分离物抗性相关的qtl。在ChLCV-Raichur分离物抗性相关的第6染色体上定位了一个显著的QTL。在鉴定的位点内预测防御相关基因。在IHR4615(R) × IHR2451(S) F2群体中，进一步用SSR标记对鉴定位点进行细化，鉴定出与ChLCV-Raichur分离物抗性相关的两个SSR标记IHR-LCV-SSR-76和IHR-LCV-SSR-165，预测效率分别为89.50%和72.50% %。这些标记与抗性基因的遗传距离分别为7 cM和17.65 cM。这些标记在另一个基于抗性源的IHR4392(S) × IHR4597(R) F2群体中得到进一步验证。开发和验证的分子标记可用于辣椒抗病品种/ F1杂交种的标记辅助育种。

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Molecular mapping and development of SSR markers associated with Chilli leaf curl virus resistance in chilli (Capsicum annuum L.)

Chilli leaf curl virus (ChLCV), caused by whiteflies transmitted begomoviruses, poses a significant threat to chilli cultivation and production all over the world. Exploring host plant resistance and identification of associated molecular markers will accelerate resistance breeding. QTL-seq analysis was employed in the IHR4615(R) × IHR2451(S) F₂ population to identify QTLs associated with ChLCV-Raichur isolate resistance. A significant QTL was mapped on chromosome 6 associated with ChLCV-Raichur isolate resistance. Defense-related genes were predicted within the identified locus. Further refining of the identified locus with simple sequence repeats (SSR) markers led to the identification of two SSR markers IHR-LCV-SSR-76 and IHR-LCV-SSR-165 associated with the ChLCV-Raichur isolate resistance with 89.50 and 72.50 % prediction efficacy, respectively in IHR4615(R) × IHR2451(S) F₂ population. These markers are located at 7 cM and 17.65 cM genetic distances from the resistant gene. These markers were further validated in another resistant source-based F₂ population of IHR4392(S) × IHR4597(R). The developed and validated molecular markers can be explored in marker-assisted breeding programs aiming at developing resistant cultivars/ F₁ hybrids of chilli.

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.