Dorsomorphin （DM）通过作用于BMP信号通路抑制三瘤梭鱼卵巢发育

IF 2.2 2区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2025-02-11 DOI:10.1016/j.cbd.2025.101440

Xiaocong Chen , Ce Shi , Yangfang Ye , Chunlin Wang , Ronghua Li , Huan Wang , Congcong Hou , Weiwei Song , Xinghong Xu , Changkao Mu

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引用次数: 0

摘要

骨形态发生蛋白（BMPs）调节动物生长、细胞增殖和分化。BMP信号通路在哺乳动物卵泡发育过程中起着重要的调节作用。然而，对甲壳类动物的相关研究有限。本研究的重点是BMP信号通路的关键基因，BMP I型受体。将不同浓度的dorsomorphin （DM）注射给三瘤虎（Portunus trituberculatus），观察一个月，以确定干扰BMP信号通路的最佳有效浓度。随后进行转录组学、蛋白质组学和代谢组学测量，以确定BMP信号对三瘤假单胞虫卵巢发育的影响。通过多组学分析和脂质分析，初步揭示了BMP信号通路在卵巢发育调控中的作用机制。本研究为进一步探索甲壳类动物性腺发育的分子调控机制提供了理论基础。

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Dorsomorphin (DM) inhibits the ovarian development of Portunus trituberculatus by acting on the BMP signaling pathway

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Dorsomorphin (DM) inhibits the ovarian development of Portunus trituberculatus by acting on the BMP signaling pathway

Bone morphogenic proteins (BMPs) regulate animal growth, cell proliferation and differentiation. The BMP signaling pathway plays an important regulatory role during ovarian follicle development in mammals. However, related studies in crustaceans are limited. The focus of this study was the key gene of the BMP signaling pathway, the BMP type I receptor. Portunus trituberculatus was injected with different concentrations of dorsomorphin (DM) and observed for one month to identify the optimal effective concentration for interference with the BMP signaling pathway. Subsequent transcriptomics, proteomics, and metabolomics measurements were performed to identify the effects of BMP signaling on ovarian development in P. trituberculatus. A preliminary mechanism of action of the BMP signaling pathway in the regulation of ovarian development was revealed through combined multiomics analysis and lipid analysis. This study provides a theoretical basis for further exploration of the molecular mechanism regulating gonadal development in crustaceans.

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来源期刊

Comparative Biochemistry and Physiology D-Genomics & Proteomics 生物-生化与分子生物学

CiteScore

5.10

自引率

3.30%

发文量

审稿时长

33 days

期刊介绍： Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology. Part D: Genomics and Proteomics (CBPD), focuses on “omics” approaches to physiology, including comparative and functional genomics, metagenomics, transcriptomics, proteomics, metabolomics, and lipidomics. Most studies employ “omics” and/or system biology to test specific hypotheses about molecular and biochemical mechanisms underlying physiological responses to the environment. We encourage papers that address fundamental questions in comparative physiology and biochemistry rather than studies with a focus that is purely technical, methodological or descriptive in nature.