利用光学镊子对细胞力学进行稳健量化。

IF 2.4 Q3 BIOPHYSICS
Biophysical reports Pub Date : 2025-03-12 Epub Date: 2025-02-11 DOI:10.1016/j.bpr.2025.100199
Wessel S Rodenburg, Sven F A Ebben, Jorine M Eeftens
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引用次数: 0

摘要

细胞的力学特性与功能密切相关,在许多细胞过程中起着至关重要的作用,包括迁移、分化和细胞命运的决定。已经开发了许多方法来评估各种条件下的细胞力学,但它们通常缺乏与生物学相关的皮牛顿范围力的准确性,或者对施加力的控制有限。在这里,我们提出了一种直接的方法,使用光学捕获聚苯乙烯珠来精确地将皮牛顿范围的力施加到粘附和悬浮细胞上。我们通过力反馈系统精确地对细胞施加恒定的力,允许从单个测量中量化变形,细胞刚度和蠕变响应。使用药物诱导的细胞骨架的扰动,我们表明,这种方法是敏感的检测细胞力学性能的变化。总的来说,我们提供了一个使用光学镊子对粘附和悬浮细胞施加高精度力的框架,并描述了量化细胞力学性能的直接指标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Robust quantification of cellular mechanics using optical tweezers.

The mechanical properties of cells are closely related to function and play a crucial role in many cellular processes, including migration, differentiation, and cell fate determination. Numerous methods have been developed to assess cell mechanics under various conditions, but they often lack accuracy on biologically relevant piconewton-range forces or have limited control over the applied force. Here, we present a straightforward approach for using optically trapped polystyrene beads to accurately apply piconewton-range forces to adherent and suspended cells. We precisely apply a constant force to cells by means of a force-feedback system, allowing for quantification of deformation, cell stiffness, and creep response from a single measurement. Using drug-induced perturbations of the cytoskeleton, we show that this approach is sensitive to detecting changes in cellular mechanical properties. Collectively, we provide a framework for using optical tweezers to apply highly accurate forces to adherent and suspended cells and describe straightforward metrics to quantify cellular mechanical properties.

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来源期刊
Biophysical reports
Biophysical reports Biophysics
CiteScore
2.40
自引率
0.00%
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审稿时长
75 days
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