可能的胎牛血清减少促进培养东风-1补充植物蛋白胨和酵母提取物

IF 2.4 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY
Juah Chung, Bumjin Joo, Yu Kyong Woo, Jihyun Kim, Young-oh Lee, Seokwon Lim, Choongjin Ban
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引用次数: 0

摘要

为了降低胎牛血清(FBS),促进DF-1的增殖,在培养基中加入了3种植物蛋白胨和3种酵母提取物。培养基中FBS的浓度在0 ~ 10%之间变化,并在预先确定的范围内评估细胞活力的增强情况。在每个特定浓度范围内,与对照组相比,添加物提高了活力。其中,含6.25 mg/mL小麦蛋白胨和1.25% FBS的培养基显著提高了细胞活力。这种有益效果可归因于通过补充适当的游离氨基酸(FAAs),肽,碳水化合物(不包括游离糖)或微量元素的掺入。关于FAAs,谷氨酸、谷氨酰胺、丙氨酸和非必需FAAs对细胞增殖的增强取决于补充的具体类型。丝氨酸、组氨酸、苏氨酸、精氨酸、酪氨酸、缬氨酸、蛋氨酸、苯丙氨酸、异亮氨酸、亮氨酸、必需FAAs,尤其是赖氨酸都能抑制细胞增殖。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Possible fetal bovine serum-reduced boost cultivation of DF-1 by supplementation with plant peptones and yeast extracts

To reduce the fetal bovine serum (FBS) and enhance the DF-1 proliferation, three plant peptones and three yeast extracts were incorporated into culture medium. The concentration of FBS in the medium varied from 0 to 10%, and the enhanced cell viability was evaluated within the predetermined range for supplementation. In each specific concentration range, the viability was enhanced by the supplementation compared to the control group. In particular, the medium containing 6.25 mg/mL wheat peptone and 1.25% FBS demonstrated a significant increase in viability. This beneficial effect can be attributed to the incorporation of suitable free amino acids (FAAs), peptides, carbohydrates (excluding free sugars), or trace elements through supplementation. Regarding FAAs, the enhanced cell proliferation by glutamate, glutamine, alanine, and non-essential FAAs was contingent upon the specific type of supplementation. The proliferation was suppressed by serine, histidine, threonine, arginine, tyrosine, valine, methionine, phenylalanine, isoleucine, leucine, essential FAAs, and particularly lysine.

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来源期刊
Food Science and Biotechnology
Food Science and Biotechnology FOOD SCIENCE & TECHNOLOGY-
CiteScore
5.40
自引率
3.40%
发文量
174
审稿时长
2.3 months
期刊介绍: The FSB journal covers food chemistry and analysis for compositional and physiological activity changes, food hygiene and toxicology, food microbiology and biotechnology, and food engineering involved in during and after food processing through physical, chemical, and biological ways. Consumer perception and sensory evaluation on processed foods are accepted only when they are relevant to the laboratory research work. As a general rule, manuscripts dealing with analysis and efficacy of extracts from natural resources prior to the processing or without any related food processing may not be considered within the scope of the journal. The FSB journal does not deal with only local interest and a lack of significant scientific merit. The main scope of our journal is seeking for human health and wellness through constructive works and new findings in food science and biotechnology field.
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