Ting Liu, Yun Liang, Yan Zhu, Lijun Zhang and Haibing Liao*,
{"title":"本科生跨学科实验:柱状蛋白对家蚕的拒食活性研究","authors":"Ting Liu, Yun Liang, Yan Zhu, Lijun Zhang and Haibing Liao*, ","doi":"10.1021/acs.jchemed.4c0099910.1021/acs.jchemed.4c00999","DOIUrl":null,"url":null,"abstract":"<p >Due to the time-intensive nature of isolation and purification, the low yield of pure compound, and the high costs associated with bioactivity evaluation <i>in vitro</i> and <i>in vivo</i>, comprehensive processes integrating natural product extraction, isolation, structure identification, and bioactivity evaluation <i>in vivo</i> are relatively rarely implemented in undergraduate chemistry laboratory curricula at present. Based on the teaching experience of the chromatography experimental course “Extraction and Determination of Columbin Content in <i>Tinospora Radix</i>” and the related research progress, we herein introduce a new interdisciplinary and comprehensive chemistry experiment, comprising a total of 20 lab sessions, each lasting 45 min. These experiments include the extraction of Columbin from <i>Tinospora Radix</i>, the identification of its chemical structure, and the evaluation of the antifeedant activity against the third instar silkworms. Students are actively engaged in the process of discovering active compounds from natural plants through this interdisciplinary experiment, providing them with hands-on laboratory experience in solving real-life food security challenges. The experiment adopts a multi-dimensional evaluation system including three dimensions of teacher evaluation, intragroup evaluation, and intergroup evaluation. This approach mitigates the limitation of relying on a single evaluation subject, ensures more objective evaluation results, and enhances the overall effectiveness of teaching activities. This experiment not only cultivates their comprehensive experimental “hard skills” in organic chemistry, analytical chemistry, and chemical biology but also develops their “soft skills”, such as critical thinking, teamwork, and innovation. Finally, the implementation of the experiment is characterized by the easy availability of materials, low cost, and straightforward replication, making it well-suited for senior undergraduate students in chemistry-related majors across different educational levels.</p>","PeriodicalId":43,"journal":{"name":"Journal of Chemical Education","volume":"102 2","pages":"787–795 787–795"},"PeriodicalIF":2.9000,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"An Interdisciplinary Experiment for Undergraduates: A Study of the Antifeedant Activity of Columbin on the Silkworm\",\"authors\":\"Ting Liu, Yun Liang, Yan Zhu, Lijun Zhang and Haibing Liao*, \",\"doi\":\"10.1021/acs.jchemed.4c0099910.1021/acs.jchemed.4c00999\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Due to the time-intensive nature of isolation and purification, the low yield of pure compound, and the high costs associated with bioactivity evaluation <i>in vitro</i> and <i>in vivo</i>, comprehensive processes integrating natural product extraction, isolation, structure identification, and bioactivity evaluation <i>in vivo</i> are relatively rarely implemented in undergraduate chemistry laboratory curricula at present. Based on the teaching experience of the chromatography experimental course “Extraction and Determination of Columbin Content in <i>Tinospora Radix</i>” and the related research progress, we herein introduce a new interdisciplinary and comprehensive chemistry experiment, comprising a total of 20 lab sessions, each lasting 45 min. These experiments include the extraction of Columbin from <i>Tinospora Radix</i>, the identification of its chemical structure, and the evaluation of the antifeedant activity against the third instar silkworms. Students are actively engaged in the process of discovering active compounds from natural plants through this interdisciplinary experiment, providing them with hands-on laboratory experience in solving real-life food security challenges. The experiment adopts a multi-dimensional evaluation system including three dimensions of teacher evaluation, intragroup evaluation, and intergroup evaluation. This approach mitigates the limitation of relying on a single evaluation subject, ensures more objective evaluation results, and enhances the overall effectiveness of teaching activities. This experiment not only cultivates their comprehensive experimental “hard skills” in organic chemistry, analytical chemistry, and chemical biology but also develops their “soft skills”, such as critical thinking, teamwork, and innovation. Finally, the implementation of the experiment is characterized by the easy availability of materials, low cost, and straightforward replication, making it well-suited for senior undergraduate students in chemistry-related majors across different educational levels.</p>\",\"PeriodicalId\":43,\"journal\":{\"name\":\"Journal of Chemical Education\",\"volume\":\"102 2\",\"pages\":\"787–795 787–795\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-01-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Chemical Education\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acs.jchemed.4c00999\",\"RegionNum\":3,\"RegionCategory\":\"教育学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Chemical Education","FirstCategoryId":"92","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.jchemed.4c00999","RegionNum":3,"RegionCategory":"教育学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
An Interdisciplinary Experiment for Undergraduates: A Study of the Antifeedant Activity of Columbin on the Silkworm
Due to the time-intensive nature of isolation and purification, the low yield of pure compound, and the high costs associated with bioactivity evaluation in vitro and in vivo, comprehensive processes integrating natural product extraction, isolation, structure identification, and bioactivity evaluation in vivo are relatively rarely implemented in undergraduate chemistry laboratory curricula at present. Based on the teaching experience of the chromatography experimental course “Extraction and Determination of Columbin Content in Tinospora Radix” and the related research progress, we herein introduce a new interdisciplinary and comprehensive chemistry experiment, comprising a total of 20 lab sessions, each lasting 45 min. These experiments include the extraction of Columbin from Tinospora Radix, the identification of its chemical structure, and the evaluation of the antifeedant activity against the third instar silkworms. Students are actively engaged in the process of discovering active compounds from natural plants through this interdisciplinary experiment, providing them with hands-on laboratory experience in solving real-life food security challenges. The experiment adopts a multi-dimensional evaluation system including three dimensions of teacher evaluation, intragroup evaluation, and intergroup evaluation. This approach mitigates the limitation of relying on a single evaluation subject, ensures more objective evaluation results, and enhances the overall effectiveness of teaching activities. This experiment not only cultivates their comprehensive experimental “hard skills” in organic chemistry, analytical chemistry, and chemical biology but also develops their “soft skills”, such as critical thinking, teamwork, and innovation. Finally, the implementation of the experiment is characterized by the easy availability of materials, low cost, and straightforward replication, making it well-suited for senior undergraduate students in chemistry-related majors across different educational levels.
期刊介绍:
The Journal of Chemical Education is the official journal of the Division of Chemical Education of the American Chemical Society, co-published with the American Chemical Society Publications Division. Launched in 1924, the Journal of Chemical Education is the world’s premier chemical education journal. The Journal publishes peer-reviewed articles and related information as a resource to those in the field of chemical education and to those institutions that serve them. JCE typically addresses chemical content, activities, laboratory experiments, instructional methods, and pedagogies. The Journal serves as a means of communication among people across the world who are interested in the teaching and learning of chemistry. This includes instructors of chemistry from middle school through graduate school, professional staff who support these teaching activities, as well as some scientists in commerce, industry, and government.