本科生跨学科实验:柱状蛋白对家蚕的拒食活性研究

IF 2.9 3区 教育学 Q2 CHEMISTRY, MULTIDISCIPLINARY
Ting Liu, Yun Liang, Yan Zhu, Lijun Zhang and Haibing Liao*, 
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引用次数: 0

摘要

由于分离纯化耗时长,纯化合物收率低,体外和体内生物活性评价成本高,目前在本科化学实验课程中,整合天然产物提取、分离、结构鉴定和体内生物活性评价的综合过程相对较少。根据色谱实验课程“天竺草中柱状蛋白含量的提取与测定”的教学经验和相关研究进展,我们推出了一个新的跨学科综合化学实验,共20个实验课时,每个实验课时45 min。实验内容包括天竺草中柱状蛋白的提取、化学结构的鉴定、并对三龄家蚕进行了拒食活性评价。通过这个跨学科的实验,学生们积极参与到从天然植物中发现活性化合物的过程中,为他们提供解决现实生活中食品安全挑战的动手实验室经验。实验采用多维度评价体系,包括教师评价、组内评价和组间评价三个维度。这种方法减轻了依赖单一评价主体的局限性,保证了评价结果更加客观,提高了教学活动的整体有效性。本实验不仅培养了学生在有机化学、分析化学、化学生物学等方面的综合实验“硬技能”,也培养了学生的批判性思维、团队合作、创新能力等“软技能”。最后,实验的实施具有材料易得、成本低、复制简单等特点,适合不同学历的化学相关专业本科高年级学生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

An Interdisciplinary Experiment for Undergraduates: A Study of the Antifeedant Activity of Columbin on the Silkworm

An Interdisciplinary Experiment for Undergraduates: A Study of the Antifeedant Activity of Columbin on the Silkworm

Due to the time-intensive nature of isolation and purification, the low yield of pure compound, and the high costs associated with bioactivity evaluation in vitro and in vivo, comprehensive processes integrating natural product extraction, isolation, structure identification, and bioactivity evaluation in vivo are relatively rarely implemented in undergraduate chemistry laboratory curricula at present. Based on the teaching experience of the chromatography experimental course “Extraction and Determination of Columbin Content in Tinospora Radix” and the related research progress, we herein introduce a new interdisciplinary and comprehensive chemistry experiment, comprising a total of 20 lab sessions, each lasting 45 min. These experiments include the extraction of Columbin from Tinospora Radix, the identification of its chemical structure, and the evaluation of the antifeedant activity against the third instar silkworms. Students are actively engaged in the process of discovering active compounds from natural plants through this interdisciplinary experiment, providing them with hands-on laboratory experience in solving real-life food security challenges. The experiment adopts a multi-dimensional evaluation system including three dimensions of teacher evaluation, intragroup evaluation, and intergroup evaluation. This approach mitigates the limitation of relying on a single evaluation subject, ensures more objective evaluation results, and enhances the overall effectiveness of teaching activities. This experiment not only cultivates their comprehensive experimental “hard skills” in organic chemistry, analytical chemistry, and chemical biology but also develops their “soft skills”, such as critical thinking, teamwork, and innovation. Finally, the implementation of the experiment is characterized by the easy availability of materials, low cost, and straightforward replication, making it well-suited for senior undergraduate students in chemistry-related majors across different educational levels.

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来源期刊
Journal of Chemical Education
Journal of Chemical Education 化学-化学综合
CiteScore
5.60
自引率
50.00%
发文量
465
审稿时长
6.5 months
期刊介绍: The Journal of Chemical Education is the official journal of the Division of Chemical Education of the American Chemical Society, co-published with the American Chemical Society Publications Division. Launched in 1924, the Journal of Chemical Education is the world’s premier chemical education journal. The Journal publishes peer-reviewed articles and related information as a resource to those in the field of chemical education and to those institutions that serve them. JCE typically addresses chemical content, activities, laboratory experiments, instructional methods, and pedagogies. The Journal serves as a means of communication among people across the world who are interested in the teaching and learning of chemistry. This includes instructors of chemistry from middle school through graduate school, professional staff who support these teaching activities, as well as some scientists in commerce, industry, and government.
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