Helen Bachmann, Robrecht Raedt, Guy Laureys, Kristl Vonck
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引用次数: 0
摘要
喉肌诱发电位(LMEP)是迷走神经刺激(VNS)实验中保证神经电极界面完整性的神经生理学结果参数。本文讨论了46只雌性Lewis大鼠在左侧颈迷走神经周围植入定制的VNS电极后的大量微创LMEP记录。恢复3周后,每只动物记录两次lmep,交换VNS电极的阳极和阴极位置(极性反转)。vns诱发的LMEP被确定为刺激后的初始负峰波伪影,在给定的刺激输出电流下的所有扫描中都一致记录。潜伏期定义为从刺激开始到这个负峰的时间,刺激阈值定义为显示清晰且可重复的LMEP的最低电流。46只动物中有37只(80.4%)出现LMEP反应,刺激强度阈值为0.37±0.27 mA,潜伏期为2.39±0.45 ms。在尾部电极接触处首先施加阴极脉冲相位导致LMEP潜伏期最短(MWU: p = 0.049)。(2.36±0.43 ms vs. 2.41±0.47 ms)。微创LMEP记录为检查电极功能和正确植入提供了可行可靠的手段。
Use of laryngeal muscle evoked potential recording for experimental vagus nerve stimulation
The laryngeal muscle evoked potential (LMEP) is a neurophysiological outcome parameter that guarantees integrity of the nerve-electrode interface during experiments with vagus nerve stimulation (VNS). This paper discusses a large series of minimally invasive LMEP recordings in 46 female Lewis rats, implanted with a custom-made VNS electrode around the left cervical vagus nerve. After a 3-week recovery, LMEPs were recorded twice in each animal, with swapping the anode and cathode positions of the VNS electrode (polarity inversion). A VNS-induced LMEP was identified as the initial negative peak wave post-stimulation artifact, consistently recorded in all sweeps at a given stimulation output current. Latency was defined as the time from stimulation onset to this negative peak, and stimulation threshold as the lowest current showing a clear and reproducible LMEP. An LMEP response was shown by 37/46 animals (80.4%), with stimulation intensity threshold of 0.37 ± 0.27 mA and latency of 2.39 ± 0.45 ms. Administering the cathodic pulse phase first at the caudal electrode contact resulted in the shortest LMEP latencies (MWU: p = 0.049. 2.36 ± 0.43 ms vs. 2.41 ± 0.47 ms). Minimally invasive LMEP recording provides a feasible and reliable means for checking electrode functioning and correct implantation.