微和纳米聚苯乙烯塑料颗粒对斑马鱼幼鱼的表型和行为毒性评价。

IF 4.1 3区 医学 Q2 TOXICOLOGY
Bailey Levesque, Sabahudin Hrapovic, Fabrice Berrué, Anja Vogt, Lee D Ellis, Ludovic Hermabessiere
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引用次数: 0

摘要

在所有环境中都发现了塑料颗粒,有必要了解这些颗粒对环境构成的风险。这项工作的目的是了解不同大小和浓度的聚苯乙烯(PS)微塑料和纳米塑料对斑马鱼胚胎的毒性作用及其在幼虫体内的命运。在这项工作中,斑马鱼(Danio rerio)幼虫暴露于6种尺寸(0.05,0.25,0.53,2.1,6.02和10.2µm直径)和浓度(0.0005-0.2µg/µL)的PS微/纳米塑料颗粒中。采用斑马鱼胚胎毒性(ZET)和一般毒性和行为毒性(GBT)试验测定颗粒在胚胎中的毒性。进行行为分析,并评估微/纳米塑料的摄取和器官分布。除0.25µm颗粒暴露的幼虫外,所有暴露均观察到表型和行为毒性。在测试的最高暴露浓度下观察到显著的表型毒性,随着时间的增加,一些大小显示出潜在的恢复。行为分析表明,所有微和纳米塑料尺寸的基线活性都有所下降。在ZET试验中,较小尺寸的颗粒的明暗反应显著增加,而在较大尺寸的颗粒中没有观察到显著的影响。除0.05µm颗粒外,在所有测试尺寸的GBT试验中,这种反应都显着降低。这些试验证明了微/纳米塑料对模式生物的一般、发育和神经毒性,可用于推断个体和群体水平的暴露效应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of phenotypic and behavioral toxicity of micro- and nano-plastic polystyrene particles in larval zebrafish (Danio rerio).

Plastic particles have been found in all environments and it is necessary to understand the risks these particles pose in, and to, the environment. The objectives of this work were to understand the toxic effects of varying size and concentration of polystyrene (PS) micro- and nano-plastics in zebrafish embryos and their fate within the larvae. In this work, larval zebrafish (Danio rerio) were exposed to six sizes (0.05, 0.25, 0.53, 2.1, 6.02, and 10.2 µm diameter) and concentrations (0.0005 to 0.2 µg/µL) of PS micro/nanoplastics particles. The zebrafish embryo toxicity (ZET) and the general and behavioral toxicity (GBT) assays were used to determine particle toxicity in embryos. Behavioral analysis was performed and micro/nanoplastics uptake and organ distribution were assessed. Phenotypic and behavioral toxicity was observed in all exposures with the exception of 0.25 µm particle-exposed larvae. Significant phenotypic toxicity was seen at the highest tested exposure concentration, with some sizes showing potential recovery as time increased in the assay. Behavioral analysis demonstrated a decrease in baseline activity across all micro- and nano-plastic sizes. Significant increases in light-dark responses were recorded in ZET assays of smaller-sized particles and no significant effects were observed at larger sizes. Significant decreases in this response were reported in the GBT assays of all tested sizes with the exception of the 0.05-µm particles. These assays demonstrate the general, developmental, and neurotoxicity of micro/nanoplastics to a model organism, which can be used to infer individual and population-level effects of exposure.

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来源期刊
Toxicological Sciences
Toxicological Sciences 医学-毒理学
CiteScore
7.70
自引率
7.90%
发文量
118
审稿时长
1.5 months
期刊介绍: The mission of Toxicological Sciences, the official journal of the Society of Toxicology, is to publish a broad spectrum of impactful research in the field of toxicology. The primary focus of Toxicological Sciences is on original research articles. The journal also provides expert insight via contemporary and systematic reviews, as well as forum articles and editorial content that addresses important topics in the field. The scope of Toxicological Sciences is focused on a broad spectrum of impactful toxicological research that will advance the multidisciplinary field of toxicology ranging from basic research to model development and application, and decision making. Submissions will include diverse technologies and approaches including, but not limited to: bioinformatics and computational biology, biochemistry, exposure science, histopathology, mass spectrometry, molecular biology, population-based sciences, tissue and cell-based systems, and whole-animal studies. Integrative approaches that combine realistic exposure scenarios with impactful analyses that move the field forward are encouraged.
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