亚油酸代谢与C57BL/6小鼠肌动蛋白的抗肥胖作用有关。

IF 5.3 2区医学 Q1 PHARMACOLOGY & PHARMACY

Biochemical pharmacology Pub Date : 2025-02-06 DOI:10.1016/j.bcp.2025.116793

Juan Chen , Jing-jing Yuan , Li-na Huang , Qiang-qiang Shi , Xian Zhang , Ming-hua Qiu , Jian Liu

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引用次数: 0

摘要

众所周知，天然产物是代谢疾病潜在化合物的丰富来源。在本研究中，我们旨在鉴定具有抗脂肪和抗肥胖作用的慈母菇（Cimicifuga foetida L.）的先导化合物。从CF （ECF）乙酸乙酯（EtOAc）提取物中分离出5种高含量化学物质，研究其对3个 T3-L1前脂肪细胞脂肪形成的影响。Actein在3 T3-L1脂肪细胞中具有显著的抗脂肪生成活性，其抗脂肪生成作用贯穿于整个脂肪细胞分化阶段。膳食活性蛋白改善饮食诱导的肥胖，改善紊乱的血清脂质水平，并增强饮食诱导的肥胖小鼠的白色脂肪褐变。利用小鼠尿液和血清样本进行代谢组学分析发现，肌动蛋白治疗可逆转肥胖小鼠体内γ-亚麻酸和卵磷脂的失调，从而改善高脂肪饮食引起的亚油酸代谢紊乱，这表明肌动蛋白可作为先导化合物，具有开发肥胖症及相关代谢紊乱新疗法的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Linoleic acid metabolism is implicated in the anti-obesity effects of actein in C57BL/6 mice

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Linoleic acid metabolism is implicated in the anti-obesity effects of actein in C57BL/6 mice

Natural products are known to be a rich source of potential compounds for metabolic diseases. In this study, we aim to identify the lead compounds with anti-adipogenic and anti-obesity effects in Cimicifuga foetida L. (CF). Five high-content chemicals from ethyl acetate extract of CF (ECF) were isolated to investigate their effects on adipogenesis in 3T3-L1 preadipocytes. Actein is identified to possess substantial anti-adipogenic activity in 3T3-L1 adipocytes, which exerts its anti-adipogenic effect throughout the entire adipocyte differentiation stages. Dietary actein ameliorates diet-induced obesity, improves the disturbed serum lipid levels, and enhances white fat browning in diet-induced obese mice. Metabolomic analysis using urine and serum samples from mice revealed that actein treatment reverses the dysregulation of γ-linolenic acid and lecithin in obese mice, thus ameliorating the disturbance of linoleic acid metabolism induced by high-fat diet, suggesting that actein can be used as a lead compound with the potential to develop new therapies for obesity and related metabolic disorders.

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来源期刊

Biochemical pharmacology 医学-药学

CiteScore

10.30

自引率

1.70%

发文量

420

审稿时长

17 days

期刊介绍： Biochemical Pharmacology publishes original research findings, Commentaries and review articles related to the elucidation of cellular and tissue function(s) at the biochemical and molecular levels, the modification of cellular phenotype(s) by genetic, transcriptional/translational or drug/compound-induced modifications, as well as the pharmacodynamics and pharmacokinetics of xenobiotics and drugs, the latter including both small molecules and biologics. The journal''s target audience includes scientists engaged in the identification and study of the mechanisms of action of xenobiotics, biologics and drugs and in the drug discovery and development process. All areas of cellular biology and cellular, tissue/organ and whole animal pharmacology fall within the scope of the journal. Drug classes covered include anti-infectives, anti-inflammatory agents, chemotherapeutics, cardiovascular, endocrinological, immunological, metabolic, neurological and psychiatric drugs, as well as research on drug metabolism and kinetics. While medicinal chemistry is a topic of complimentary interest, manuscripts in this area must contain sufficient biological data to characterize pharmacologically the compounds reported. Submissions describing work focused predominately on chemical synthesis and molecular modeling will not be considered for review. While particular emphasis is placed on reporting the results of molecular and biochemical studies, research involving the use of tissue and animal models of human pathophysiology and toxicology is of interest to the extent that it helps define drug mechanisms of action, safety and efficacy.