原代NK细胞的分离、鉴定及KIR2DL1+群体的富集。

4区生物学 Q4 Biochemistry, Genetics and Molecular Biology

Methods in cell biology Pub Date : 2025-01-01 Epub Date: 2024-03-02 DOI:10.1016/bs.mcb.2023.09.001

Batel Sabag, Abhishek Puthenveetil, Mira Barda-Saad

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引用次数: 0

摘要

自然杀伤细胞（NK）是具有细胞毒性的先天淋巴样细胞，通过分泌细胞溶解颗粒和免疫调节细胞因子，在减轻病毒感染和癌症中发挥关键作用。NK功能异常可导致病毒感染、自身免疫和癌症。目前的方案提供了一种NK分离技术来研究杀伤细胞免疫球蛋白样受体（KIR）下游的信号通路，KIR作为关键的人类NK细胞功能调节剂。这一过程能够研究特定于个体kir的机制，以提高我们对NK细胞在健康和疾病中的功能的理解。

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Isolation and characterization of primary NK cells and the enrichment of the KIR2DL1⁺ population.

Natural killer (NK) cells are cytotoxic innate lymphoid cells that play critical roles in the mitigation of viral infections and cancer through the secretion of cytolytic granules and immunomodulatory cytokines. Abnormalities in NK function can lead to viral infections, autoimmunity, and cancer. The current protocol provides an NK isolation technique to study the signaling pathways downstream to the Killer cell immunoglobulin-like receptors (KIR) that serve as key human NK cell function regulators. This procedure enables investigating mechanisms specific to individual KIRs to improve our understanding of NK cell function in health and disease.

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来源期刊

Methods in cell biology 生物-细胞生物学

CiteScore

3.10

自引率

0.00%

发文量

125

审稿时长

3 months

期刊介绍： For over fifty years, Methods in Cell Biology has helped researchers answer the question "What method should I use to study this cell biology problem?" Edited by leaders in the field, each thematic volume provides proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies. Over its many years of publication, Methods in Cell Biology has built up a deep library of biological methods to study model developmental organisms, organelles and cell systems, as well as comprehensive coverage of microscopy and other analytical approaches.