Luis O. Burzio , Monica Brito , Ana Maria Zarraga , M.A.Q. Siddiqui
{"title":"高效液相色谱法测定氯霉素乙酰转移酶含量","authors":"Luis O. Burzio , Monica Brito , Ana Maria Zarraga , M.A.Q. Siddiqui","doi":"10.1016/0735-0651(88)90020-9","DOIUrl":null,"url":null,"abstract":"<div><p>A procedure to measure chloramphenicol acetyl transferase (CAT) activity by reverse-phase high-performance liquid chromotography is described. The antibiotic as well as the acetylated derivatives are well resolved on a Superspher RP-18 column using equal parts of acetonitrile and 10 mM sodium acetate (ph 5.0) as a solvent. Under these conditions, less than 100 pmol of each derivative can be easily detected within 10 minutes, and no radioactive chloramphenicol is needed. The present procedure has been used to measure the activity of the enzyme in extracts of chicken fibroblast transfected with the recombinant plasmid pSV2-cat containing the CAT gene.</p></div>","PeriodicalId":77714,"journal":{"name":"Gene analysis techniques","volume":"5 1","pages":"Pages 5-8"},"PeriodicalIF":0.0000,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0735-0651(88)90020-9","citationCount":"4","resultStr":"{\"title\":\"Assay of chloramphenicol acetyl transferase by high-performance liquid chromatography\",\"authors\":\"Luis O. Burzio , Monica Brito , Ana Maria Zarraga , M.A.Q. Siddiqui\",\"doi\":\"10.1016/0735-0651(88)90020-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A procedure to measure chloramphenicol acetyl transferase (CAT) activity by reverse-phase high-performance liquid chromotography is described. The antibiotic as well as the acetylated derivatives are well resolved on a Superspher RP-18 column using equal parts of acetonitrile and 10 mM sodium acetate (ph 5.0) as a solvent. Under these conditions, less than 100 pmol of each derivative can be easily detected within 10 minutes, and no radioactive chloramphenicol is needed. The present procedure has been used to measure the activity of the enzyme in extracts of chicken fibroblast transfected with the recombinant plasmid pSV2-cat containing the CAT gene.</p></div>\",\"PeriodicalId\":77714,\"journal\":{\"name\":\"Gene analysis techniques\",\"volume\":\"5 1\",\"pages\":\"Pages 5-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1988-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0735-0651(88)90020-9\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gene analysis techniques\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0735065188900209\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene analysis techniques","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0735065188900209","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Assay of chloramphenicol acetyl transferase by high-performance liquid chromatography
A procedure to measure chloramphenicol acetyl transferase (CAT) activity by reverse-phase high-performance liquid chromotography is described. The antibiotic as well as the acetylated derivatives are well resolved on a Superspher RP-18 column using equal parts of acetonitrile and 10 mM sodium acetate (ph 5.0) as a solvent. Under these conditions, less than 100 pmol of each derivative can be easily detected within 10 minutes, and no radioactive chloramphenicol is needed. The present procedure has been used to measure the activity of the enzyme in extracts of chicken fibroblast transfected with the recombinant plasmid pSV2-cat containing the CAT gene.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
