硝唑啉对从中国分离的尿路病原体的体外抗菌活性。

IF 3.7 Q2 INFECTIOUS DISEASES
JAC-Antimicrobial Resistance Pub Date : 2025-02-04 eCollection Date: 2025-02-01 DOI:10.1093/jacamr/dlaf012
Xiaofei Yi, Xin Chen, Yanyan Lu, Jianfeng Zhang, Jinhong Chen, Minggui Wang, Xiaogang Xu
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引用次数: 0

摘要

背景和目的:抗菌硝基喹啉在一些欧洲国家用于治疗无并发症的尿路感染(uti)。中国泌尿系统病原菌体外抗菌数据缺乏。目的:探讨硝基喹啉对中国患者尿路病原菌的体外抑菌活性。方法:采用微量肉汤稀释法对从尿液中分离的鲍曼不动杆菌等10种229株细菌进行测定。所有分离株均采用EUCAST对大肠杆菌的药敏断点(16 mg/L)。测定了鲍曼不动杆菌(n = 34)的MBC, MBC定义为硝基喹啉浓度,在该浓度下,初始接种量减少99.9%。测定了两株鲍曼不动杆菌在24 h内的时间杀伤曲线。结果:除铜绿假单胞菌外,大多数菌株对硝基喹啉敏感。硝基喹啉对大肠杆菌、肺炎克雷伯菌和奇异变形杆菌的MIC50/90值分别为4/8、8/32和8/16 mg/L。在革兰氏阴性菌株中,硝基喹啉对鲍曼不动杆菌的抑制效果较好,其MIC50/90值为2/2 mg/L。鲍曼不动杆菌的MBC等于或比MIC高1倍。鲍曼不动杆菌的时间杀伤曲线呈浓度依赖性。结论:硝基喹啉对中国产尿路病原菌具有良好的体外活性,可能是治疗鲍曼不动杆菌引起的非复杂性尿路感染的良好选择。还需要进一步的体内疗效研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vitro antimicrobial activity of nitroxoline against uropathogens isolated from China.

Background and objectives: The antimicrobial nitroxoline is used in treating uncomplicated urinary tract infections (UTIs) in some European countries. In vitro antimicrobial data on uropathogens from China are lacking. To investigate the activity of nitroxoline in vitro against uropathogens isolated from Chinese patients.

Methods: MICs of nitroxoline were determined using broth microdilution of 229 bacterial isolates of 10 species including Acinetobacter baumannii derived from urinary sample. The EUCAST susceptibility breakpoint for Escherichia coli (16 mg/L) was applied for all isolates. The MBC for A. baumannii (n = 34) was determined, with the MBC defined as the nitroxoline concentration at which a 99.9% reduction in the initial inoculum was observed. Time-kill curves of the two isolates of A. baumannii were tested over a 24 h period.

Results: Except for Pseudomonas aeruginosa, most isolates were susceptible to nitroxoline. The MIC50/90 values of nitroxoline for E. coli, Klebsiella pneumoniae and Proteus mirabilis were 4/8, 8/32 and 8/16 mg/L, respectively. Among the Gram-negative isolates, nitroxoline demonstrated a better inhibitory effect against A. baumannii with an MIC50/90 value of 2/2 mg/L. The MBC of A. baumannii was equal to the MIC or one dilution higher. The time-kill curves of A. baumannii displayed concentration-dependent killing.

Conclusions: Nitroxoline showed excellent in vitro activity against uropathogens isolated from China and may be a good option for uncomplicated UTIs caused by A. baumannii, which are more challenging and have few clinical options. Further in vivo efficacy studies are needed.

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CiteScore
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