混合模式整体石同时富集纯化甘草查尔酮A和异甘草素

IF 1.3 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Chromatographia Pub Date : 2024-10-30 DOI:10.1007/s10337-024-04374-4

Yijie Liu, Dan Zhang, Ligai Bai, Hongyuan Yan, Haiyan Liu

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引用次数: 0

摘要

本文采用一步法在色谱柱两端分别合成亲疏水材料制备了混模单体。采用所制备的混合模式整体体作为固相萃取吸附剂，结合高效液相色谱法同时富集纯化甘草查尔酮A和异甘草素。其富集和纯化甘草查尔酮A和异尿素的能力优于单一亲水或疏水单体。方法在0.50 ~ 400 μg/mL范围内线性良好。线性回归方程为y = 465.45 ×−1.04，相关系数为0.9999。日内、日间精密度的相对标准偏差（rsd）均小于1.19%。结果表明，该方法简便、准确，可用于甘草查尔酮A和异甘草素的富集纯化。

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Simultaneous Enrichment and Purification of Licorice Chalcone A and Isoliquiritigenin in Licorice Using a Mixed-Mode Monolith

In this paper, a mixed-mode monolith was prepared by separately synthesizing hydrophilic and hydrophobic materials at both ends of a chromatography column by a one-step method. The prepared mixed mode monolith was used as solid phase extraction adsorbent for simultaneous enrichment and purification of licorice chalcone A and isoliquiritigenin from licorice combined with high performance liquid chromatography (HPLC). It had superior ability to enrich and purify licorice chalcone A and isoliquiritigenin than single hydrophilic or hydrophobic monolith. The method had good linearity in the range of 0.50–400 μg/mL. The linear regression equation was y = 465.45 × − 1.04 and the correlation coefficient was 0.9999. The relative standard deviations (RSDs) of intra-day and inter-day precision were both less than 1.19%. The results showed that the method was simple and accurate, and could be used for the enrichment and purification of licorice chalcone A and isoliquiritigenin from licorice.

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来源期刊

Chromatographia 化学-分析化学

CiteScore

3.40

自引率

5.90%

发文量

103

审稿时长

2.2 months

期刊介绍： Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.