鱼精蛋白和聚l -精氨酸对人白细胞补体C5a受体的拮抗作用。

U B Olsen, J Selmer, J U Kahl
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引用次数: 11

摘要

研究表明,鱼精蛋白选择性和剂量依赖性地抑制补体c5a诱导的白细胞反应,如嗜碱性细胞释放组胺,中性粒细胞释放化学发光和β -葡萄糖醛酸酶。鱼精蛋白使C5a剂量-反应曲线平行向右偏移。多肽的抑制能力是可逆的,并在反复洗涤暴露的细胞后消失。在中性粒细胞中,聚l -精氨酸类似地特异性拮抗c5a诱导的化学发光和酶释放。然而,这种聚合物单独可以使嗜碱性粒细胞和中性粒细胞脱粒,分别导致组胺和酶的释放。由此可见,在人中性粒细胞中,富含精氨酸的多阳离子鱼精蛋白和多l -精氨酸表现出竞争性的C5a受体拮抗作用。此外,鱼精蛋白抑制嗜碱性细胞上的C5a受体。据推测,富含精氨酸的聚阳离子的分子构象可能可逆地结合并阻断c5a受体位点的负电荷基团。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Complement C5a receptor antagonism by protamine and poly-L-Arg on human leukocytes.

It is shown that protamine selectively and dose-dependently inhibits complement C5a-induced leukocyte responses such as histamine release from basophils, chemiluminescence and beta-glucuronidase release from neutrophils. Protamine produces parallel rightward displacements of the C5a dose-response curves. The inhibitory capacity of the polypeptide is reversible and disappears following repeated washing of exposed cells. In neutrophils poly-L-Arg similarly and specifically antagonizes C5a-induced chemiluminescence and enzyme release. This polymer alone, however, degranulates basophils and neutrophils, leading to histamine and enzyme release, respectively. It is concluded that on human neutrophils the arginine-rich polycations protamine and poly-L-Arg exhibit a competitive C5a receptor antagonism. In addition, protamine inhibits the C5a receptors on basophils. It is hypothesized that molecular conformations of the arginine-rich polycations might bind reversibly to, and block negatively charged groups at the C5a-receptor sites.

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