通过基因与蛋白n端结构域之间的g -四重体特异性相互作用对TRF2的自动调节

IF 3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Xiaojuan Xu,  and , Tao Wang*, 
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引用次数: 0

摘要

端粒重复结合因子2 (TRF2)是保护端粒完整性的庇护蛋白复合物的关键组分。前面讨论的TRF2主要集中在端粒上,其他方面的讨论相对较少。研究证明,除了端粒外,TRF2还定位于整个基因组中其他可能形成g -四重体的序列。因此,除了众所周知的保护端粒的功能外,它可能普遍参与调节基因。在这里,我们证明了TRF2的n端基本结构域(TRF2B)可以与其基因的5 ' -UTR序列形成的g -四重体相互作用。随后,这种相互作用被确定为g -四重体特异性。通过一个报告基因系统,我们证明了报告基因的翻译被TRF2B和g -四重体的相互作用大大减少。总之,我们提出TRF2可以通过其自身基因序列形成的g -四重体进行“自动调节”。这一发现表明在TRF2基因的调控中存在潜在的反馈机制。此外,它还提示了一种涉及TRF2和g -四重体合作的基因调控的共同模式。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Autoregulation of TRF2 through G-Quadruplex-Specific Interaction between the Gene and N-Terminal Domain of the Protein

Autoregulation of TRF2 through G-Quadruplex-Specific Interaction between the Gene and N-Terminal Domain of the Protein

Telomere repeat-binding factor 2 (TRF2) is a key component of the shelterin complex which guards the integrity of the telomere. Most of the TRF2 discussed previously was focused on the telomere, and relatively less is discussed on aspects other than that. It is proved that TRF2 also localizes to other potential G-quadruplex-forming sequences among the whole genome besides the telomere. Therefore, it may participate in regulating genes generally except for the well-known function of protecting telomeres. Here, we demonstrate that the N-terminal basic domain of TRF2 (TRF2B) can interact with the G-quadruplex formed by the 5′-UTR sequence of its gene. Subsequently, this interaction was identified as G-quadruplex-specific. Using a reporter gene system, we proved that the translation of the reporter gene was dramatically reduced, triggered by the interaction between TRF2B and the G-quadruplex. Altogether, we propose that TRF2 can be “auto-regulated” through the G-quadruplex formed by its own gene sequence. This finding indicates a potential feedback mechanism in the regulation of the TRF2 gene. Additionally, it suggests a common mode in gene regulation involving the cooperation of TRF2 and the G-quadruplex.

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来源期刊
Biochemistry Biochemistry
Biochemistry Biochemistry 生物-生化与分子生物学
CiteScore
5.50
自引率
3.40%
发文量
336
审稿时长
1-2 weeks
期刊介绍: Biochemistry provides an international forum for publishing exceptional, rigorous, high-impact research across all of biological chemistry. This broad scope includes studies on the chemical, physical, mechanistic, and/or structural basis of biological or cell function, and encompasses the fields of chemical biology, synthetic biology, disease biology, cell biology, nucleic acid biology, neuroscience, structural biology, and biophysics. In addition to traditional Research Articles, Biochemistry also publishes Communications, Viewpoints, and Perspectives, as well as From the Bench articles that report new methods of particular interest to the biological chemistry community.
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