三维球体在缺氧和放疗相关研究中的活细胞成像和分析。

IF 2.7 3区医学 Q3 ONCOLOGY

Clinical and Translational Radiation Oncology Pub Date : 2025-01-15 DOI:10.1016/j.ctro.2025.100920

Claire Beckers, Lazaros Vasilikos, Lorena Moor, Martin Pruschy

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引用次数: 0

摘要

三维球体是研究缺氧和辐射抵抗之间相互作用的有价值的工具。然而，在球体内观察常氧和缺氧区域以及评估治疗反应的标准化方法尚缺乏。我们开发了一种简单的工作流程，用于基于图像处理的球体分析，使用一种新的定制脚本和流式细胞术。

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Live-cell imaging and analysis of 3D spheroids in hypoxia- and radiotherapy-related research

3D spheroids are a valuable tool for investigating the interplay between hypoxia and radioresistance. However, standardized methods for visualizing normoxic and hypoxic regions within spheroids and assessing treatment responses are lacking. We developed a straightforward workflow for the analysis of spheroids based on image processing using a novel custom-written script, and flow cytometry.

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