在aot -水反胶束中固定化磷脂酶A1高效生产富含dha磷脂的酶促方法的开发。

IF 3.4 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Enzyme and Microbial Technology Pub Date : 2025-01-30 DOI:10.1016/j.enzmictec.2025.110600

Qin Gao , Xiaolin Yu , Jianming Wei , Xuechao Hu , Lujing Ren

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引用次数: 0

摘要

对omega-3多不饱和脂肪酸（PUFAs），特别是二十二碳六烯酸（DHA）的需求一直在稳步增长，因为它们具有显著的健康益处。传统的生产富含dha磷脂的方法效率低、成本高。在本研究中，我们开发了一种高效的酶法制备磷脂- dha的工艺，该工艺使用固定化磷脂酶A1催化aot -水反胶束体系中的酯交换反应。最初，通过酸水解藻类油，然后结晶，产生高浓度的游离脂肪酸。在评价的6个反胶束体系中，选择1个体系进行进一步优化。采用单因素实验和响应面法对底物/酶比、温度、反应时间和水含量进行优化。为了提高成本效益和环保措施，我们实施了基材回收，以最大限度地利用基材。本研究建立了磷脂- dha制备的综合工艺链，促进了磷脂- dha的工业化生产，为其他磷脂产品的生产提供了参考。

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Development of an enzymatic method for efficient production of DHA-enriched phospholipids through immobilized phospholipase A1 in AOT-water reverse micelles

The demand for omega-3 polyunsaturated fatty acids (PUFAs), particularly docosahexaenoic acid (DHA), has been steadily increasing due to their significant health benefits. Traditional methods for producing DHA-enriched phospholipids often suffer from low efficiency and high costs. In this study, we developed an efficient enzymatic process to prepare phospholipid-DHA, which used immobilized phospholipase A1 to catalyze transesterification in AOT-water reverse micelle systems. Initially, high concentrations of free fatty acids were produced via acid hydrolysis of algae oil followed by crystallization. Among six evaluated reverse micelle systems, one was selected for further optimization. The substrate/enzyme ratio, temperature, reaction time, and water content were optimized using single-factor experiments and response surface methodology. To enhance cost-efficiency and eco-friendly practices, substrate recycling was implemented to maximize substrate utilization. This study established a comprehensive process chain for the preparation of phospholipid-DHA, promoting its industrial production and providing a reference for the production of other phospholipid products.

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来源期刊

Enzyme and Microbial Technology 生物-生物工程与应用微生物

CiteScore

7.60

自引率

5.90%

发文量

142

审稿时长

38 days

期刊介绍： Enzyme and Microbial Technology is an international, peer-reviewed journal publishing original research and reviews, of biotechnological significance and novelty, on basic and applied aspects of the science and technology of processes involving the use of enzymes, micro-organisms, animal cells and plant cells. We especially encourage submissions on: Biocatalysis and the use of Directed Evolution in Synthetic Biology and Biotechnology Biotechnological Production of New Bioactive Molecules, Biomaterials, Biopharmaceuticals, and Biofuels New Imaging Techniques and Biosensors, especially as applicable to Healthcare and Systems Biology New Biotechnological Approaches in Genomics, Proteomics and Metabolomics Metabolic Engineering, Biomolecular Engineering and Nanobiotechnology Manuscripts which report isolation, purification, immobilization or utilization of organisms or enzymes which are already well-described in the literature are not suitable for publication in EMT, unless their primary purpose is to report significant new findings or approaches which are of broad biotechnological importance. Similarly, manuscripts which report optimization studies on well-established processes are inappropriate. EMT does not accept papers dealing with mathematical modeling unless they report significant, new experimental data.