转录组学和蛋白质组学表征半马鲁肽在糖尿病认知障碍小鼠中的抗氧化机制。

IF 5.7 3区 医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
International journal of molecular medicine Pub Date : 2025-04-01 Epub Date: 2025-01-31 DOI:10.3892/ijmm.2025.5497
Ying Yang, Lulu Song, Liping Yu, Jinping Zhang, Bo Zhang
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引用次数: 0

摘要

本研究的目的是研究西马鲁肽在糖尿病相关认知能力下降(ddad)中的神经保护作用,同时探索其抗氧化作用的潜在机制。本研究使用炎症daca模型评估了semaglutide的抗氧化特性。为了研究潜在的机制,使用了组学技术。对细胞进行了全面的转录组学和蛋白质组学分析,以确定所观察到的抗氧化作用的途径。Semaglutide显示出在减轻海马病理损伤的同时增强学习和记忆功能的潜力。RNA测序和数据独立获取蛋白质组学分析确定了对照组和2型糖尿病(T2DM)组之间13,511个差异表达基因和588个差异表达蛋白。此外,1,378个基因和2,394个蛋白在T2DM和semaglutide(10µg/kg)治疗组之间表现出差异表达。转录组学和蛋白质组学的联合分析揭示了40种常见的途径。观察到酰基辅酶a氧化酶1 (ACOX1)在氧化应激期间被激活,随后被semaglutide抑制。值得注意的是,在高糖(HG)条件下,semaglutide的抗氧化和抗凋亡特性在ACOX1过表达后部分逆转。总的来说,目前的数据提供了分子证据,阐明了半马鲁肽与神经元功能之间的生理联系,并有助于阐明半马鲁肽在抗氧化应激和HG诱导的认知障碍中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Transcriptomics and proteomics characterizing the antioxidant mechanisms of semaglutide in diabetic mice with cognitive impairment.

The aim of the present study was to investigate the neuroprotective effects of semaglutide in diabetes‑associated cognitive decline (DACD), while also exploring the underlying mechanisms targeting anti‑oxidative effects. The present study evaluated the antioxidant properties of semaglutide using a DACD model of inflammation. To investigate the underlying mechanisms, omics technologies were employed. Comprehensive transcriptomic and proteomic analysis of the cells was conducted to identify the pathways responsible for the observed antioxidant effects. Semaglutide demonstrated the potential to enhance learning and memory functions while mitigating hippocampal pathological damage. RNA‑sequencing and data‑independent acquisition proteomics analyses identified 13,511 differentially expressed genes and 588 differentially expressed proteins between the control and type 2 diabetes mellitus (T2DM) groups. In addition, 1,378 genes and 2,394 proteins exhibited a differential expression between the T2DM and semaglutide (10 µg/kg) treatment groups. A combined transcriptomic and proteomic analysis unveiled 40 common pathways. Acyl‑CoA oxidase 1 (ACOX1) was observed to be activated during oxidative stress and subsequently suppressed by semaglutide. Of note, the antioxidant and anti‑apoptotic properties of semaglutide in high glucose (HG) conditions were partially reversed upon ACOX1 overexpression. Overall, the present data provided molecular evidence to elucidate the physiological connections between semaglutide and neuronal function, and contribute to clarifying the role of semaglutide in combating oxidative stress and HG‑induced cognitive impairment.

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来源期刊
International journal of molecular medicine
International journal of molecular medicine 医学-医学:研究与实验
CiteScore
12.30
自引率
0.00%
发文量
124
审稿时长
3 months
期刊介绍: The main aim of Spandidos Publications is to facilitate scientific communication in a clear, concise and objective manner, while striving to provide prompt publication of original works of high quality. The journals largely concentrate on molecular and experimental medicine, oncology, clinical and experimental cancer treatment and biomedical research. All journals published by Spandidos Publications Ltd. maintain the highest standards of quality, and the members of their Editorial Boards are world-renowned scientists.
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