{"title":"高效液相色谱- PDA检测器同时测定长效β-激动剂及其有机对应物和皮质类固醇的绿色方法。","authors":"Sweta Gupta, Sonal Desai","doi":"10.1016/j.pharma.2025.01.009","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Accurate, precise and robust HPLC method was developed for the simultaneous estimation of long-acting β2 agonist drugs (formoterol fumarate, salmeterol xinafoate and vilanterol trifenatate) along with two organic counterparts (xinafoic acid and triphenyl acetic acid) and inhaled corticosteroids (fluticasone propionate and fluticasone furoate).</p><p><strong>Material and methods: </strong>The separation was carried out using C<sub>18</sub> column (250×4.6mm, 5μ) with eluent comprising of 0.05% orthophosphoric acid: methanol: acetonitrile (40:30:30, v/v/v) at a 1mL/min flow rate.</p><p><strong>Results: </strong>Linearity was observed in the concentration ranges of 1.5-12μg/mL for formoterol fumarate, 2.82-22.6μg/mL for xinafoic acid, 6.25-50μg/mL for vilanterol, 3.12-25μg/mL for salmeterol, 2.0-15.2μg/mL for triphenyl acetic acid, 62.5-500μg/mL for fluticasone propionate and 25-200μg/mL for fluticasone furoate.</p><p><strong>Conclusion: </strong>The proposed method was accurate, precise and robust and results were within the limit as per the ICH (Q2R1) guidelines. Additionally, the proposed method was found to be green and sustainable when analysed by different green analytical tools namely GAPI, AGREE, AGREEprep and BAGI.</p>","PeriodicalId":8332,"journal":{"name":"Annales pharmaceutiques francaises","volume":" ","pages":""},"PeriodicalIF":1.0000,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Green approach for simultaneous determination of long-acting β-agonists, their organic counterparts and corticosteroids by HPLC coupled with PDA detector.\",\"authors\":\"Sweta Gupta, Sonal Desai\",\"doi\":\"10.1016/j.pharma.2025.01.009\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Accurate, precise and robust HPLC method was developed for the simultaneous estimation of long-acting β2 agonist drugs (formoterol fumarate, salmeterol xinafoate and vilanterol trifenatate) along with two organic counterparts (xinafoic acid and triphenyl acetic acid) and inhaled corticosteroids (fluticasone propionate and fluticasone furoate).</p><p><strong>Material and methods: </strong>The separation was carried out using C<sub>18</sub> column (250×4.6mm, 5μ) with eluent comprising of 0.05% orthophosphoric acid: methanol: acetonitrile (40:30:30, v/v/v) at a 1mL/min flow rate.</p><p><strong>Results: </strong>Linearity was observed in the concentration ranges of 1.5-12μg/mL for formoterol fumarate, 2.82-22.6μg/mL for xinafoic acid, 6.25-50μg/mL for vilanterol, 3.12-25μg/mL for salmeterol, 2.0-15.2μg/mL for triphenyl acetic acid, 62.5-500μg/mL for fluticasone propionate and 25-200μg/mL for fluticasone furoate.</p><p><strong>Conclusion: </strong>The proposed method was accurate, precise and robust and results were within the limit as per the ICH (Q2R1) guidelines. Additionally, the proposed method was found to be green and sustainable when analysed by different green analytical tools namely GAPI, AGREE, AGREEprep and BAGI.</p>\",\"PeriodicalId\":8332,\"journal\":{\"name\":\"Annales pharmaceutiques francaises\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2025-01-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Annales pharmaceutiques francaises\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1016/j.pharma.2025.01.009\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annales pharmaceutiques francaises","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.pharma.2025.01.009","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
Green approach for simultaneous determination of long-acting β-agonists, their organic counterparts and corticosteroids by HPLC coupled with PDA detector.
Objective: Accurate, precise and robust HPLC method was developed for the simultaneous estimation of long-acting β2 agonist drugs (formoterol fumarate, salmeterol xinafoate and vilanterol trifenatate) along with two organic counterparts (xinafoic acid and triphenyl acetic acid) and inhaled corticosteroids (fluticasone propionate and fluticasone furoate).
Material and methods: The separation was carried out using C18 column (250×4.6mm, 5μ) with eluent comprising of 0.05% orthophosphoric acid: methanol: acetonitrile (40:30:30, v/v/v) at a 1mL/min flow rate.
Results: Linearity was observed in the concentration ranges of 1.5-12μg/mL for formoterol fumarate, 2.82-22.6μg/mL for xinafoic acid, 6.25-50μg/mL for vilanterol, 3.12-25μg/mL for salmeterol, 2.0-15.2μg/mL for triphenyl acetic acid, 62.5-500μg/mL for fluticasone propionate and 25-200μg/mL for fluticasone furoate.
Conclusion: The proposed method was accurate, precise and robust and results were within the limit as per the ICH (Q2R1) guidelines. Additionally, the proposed method was found to be green and sustainable when analysed by different green analytical tools namely GAPI, AGREE, AGREEprep and BAGI.
期刊介绍：
This journal proposes a scientific information validated and indexed to be informed about the last research works in all the domains interesting the pharmacy. The original works, general reviews, the focusing, the brief notes, subjected by the best academics and the professionals, propose a synthetic approach of the last progress accomplished in the concerned sectors. The thematic Sessions and the – life of the Academy – resume the communications which, presented in front of the national Academy of pharmacy, are in the heart of the current events.
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