巴西亚马逊河流域“白色”水体中溶解有机碳及其在中性和酸性环境下的渗透调节反应

IF 1.7 3区 农林科学 Q2 FISHERIES
Helen Sadauskas-Henrique, Luciana R de Souza-Bastos, D Scott Smith, Adalberto L Val, Chris M Wood
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引用次数: 0

摘要

对刚从里约热内卢Solimoes的环中性“白水”中采集的溶解有机碳(DOC)进行光学表征,发现其芳香性较低,分子量较低,原生含量高于里约热内卢Negro的酸性“黑水”。tambaqui (Colossoma macropomum)是Serrasalmidae的典型成员,是一种典型的新热带鱼,每年在两条河流之间洄游。我们分析了在pH 7.0和pH 4.0的弱离子水中,在不存在和存在10 mg L-1里约热内卢Solimoes DOC(“SOL DOC”)的情况下,tambaqui在24小时内的离子调节反应。测量参数包括跨鳃的上皮电位(TEP)、净通量率、Na+、Cl-、氨和尿素的血浆浓度,以及鳃Na+、K+ - atp酶、H+、atp酶和碳酸酐酶活性。结果与我们之前的研究进行了比较,使用类似的方案来检查从上里约热内卢Negro的o Gabriel da Cachoeira (SGC)的黑水中收集的10 mg L-1 DOC(“SGC DOC”)的离子调节作用。在pH 7.0时,SOL DOC对鱼鳃负TEP没有影响。暴露于pH 4.0导致TEP显着去极化到正值,而SOL DOC的存在并没有改善。这与SGC DOC形成对比,后者在pH 7.0时使TEP更负,在pH 4.0时完全防止去极化。然而,SOL DOC促进了pH 7.0时Na+和Cl-的正平衡,并有助于改善pH 4.0时这些离子的负平衡。这再次与pH值为4.0时离子损失加剧的SGC DOC形成对比。SOL DOC对离子平衡的保护作用可能与提高鳃内v型H+ atp酶活性有关,与TEP无关。讨论了对两种doc的非常不同的响应,涉及它们的光学特性,它们在测试之前的存储时间(SOL)
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dissolved organic carbon from "white" waters in Amazon basin (Brazil) and the osmoregulatory responses of tambaqui (Colossoma macropomum) in neutral and acidic environments.

Optical characterization of dissolved organic carbon (DOC) freshly collected from the circumneutral "white water" of the Rio Solimoes revealed that it had lower aromaticity, lower molecular weight, and a greater autochthonous content than DOC from the acidic "black water" of the Rio Negro. The tambaqui (Colossoma macropomum), a characid member of the Serrasalmidae, is a model neotropical fish that migrates annually between the two rivers. We analysed ionoregulatory responses of the tambaqui over 24 h in ion-poor water at pH 7.0 and pH 4.0 in the absence and presence of 10 mg L-1 Rio Solimoes DOC ("SOL DOC"). Measured parameters included transepithelial potential (TEP) across the gills, net flux rates, and plasma concentrations of Na+, Cl-, ammonia, and urea, and branchial Na+, K+ -ATPase, H+, ATPase, and carbonic anhydrase activities. Results were compared to our earlier study using similar protocols to examine the ionoregulatory effects of 10 mg L-1 DOC ("SGC DOC") collected from black water at São Gabriel da Cachoeira (SGC) in the upper Rio Negro. At pH 7.0, SOL DOC had no effect on the negative TEP across the gills. Exposure to pH 4.0 caused a marked depolarization of the TEP to positive values that was not ameliorated by the presence of SOL DOC. This contrasts with SGC DOC that drove TEP more negative at pH 7.0 and fully protected against the depolarization at pH 4.0. However, SOL DOC promoted positive balance of Na+ and Cl- at pH 7.0 and helped ameliorate the negative balance of these ions seen at pH 4.0. This again contrasts with SGC DOC that exacerbated ion losses at pH 4.0. The protective effects of SOL DOC on ion balance maybe related to increased v-type H+ ATPase activity in the gills, and unrelated to TEP. The very different responses to the two DOCs are discussed with respect to their optical properties, the time that they were in storage prior to testing (SOL <1 month, SGC 2 years), and the life history of the tambaqui in the two rivers.

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来源期刊
Journal of fish biology
Journal of fish biology 生物-海洋与淡水生物学
CiteScore
4.00
自引率
10.00%
发文量
292
审稿时长
3 months
期刊介绍: The Journal of Fish Biology is a leading international journal for scientists engaged in all aspects of fishes and fisheries research, both fresh water and marine. The journal publishes high-quality papers relevant to the central theme of fish biology and aims to bring together under one cover an overall picture of the research in progress and to provide international communication among researchers in many disciplines with a common interest in the biology of fish.
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