大麦抗黄花叶病新QTL的鉴定与鉴定。

IF 3.9 2区 生物学 Q1 GENETICS & HEREDITY
Plant Genome Pub Date : 2025-03-01 DOI:10.1002/tpg2.20557
Yi Hong, Hui Zhou, Mengna Zhang, Yuhang Zhang, Juan Zhu, Chao Lv, Baojian Guo, Feifei Wang, Qingliang Li, Jie Sun, Rugen Xu
{"title":"大麦抗黄花叶病新QTL的鉴定与鉴定。","authors":"Yi Hong, Hui Zhou, Mengna Zhang, Yuhang Zhang, Juan Zhu, Chao Lv, Baojian Guo, Feifei Wang, Qingliang Li, Jie Sun, Rugen Xu","doi":"10.1002/tpg2.20557","DOIUrl":null,"url":null,"abstract":"<p><p>Winter barley (Hordeum vulgare) production areas in the middle and lower reaches of the Yangtze River are severely threatened by barley yellow mosaic disease, which is caused by Barley yellow mosaic virus and Barley mild mosaic virus. Improving barley disease resistance in breeding programs requires knowledge of genetic loci in germplasm resources. In this study, bulked segregant analysis (BSA) identified a novel major quantitative trait loci (QTL) QRym.ZN1-7H for barley yellow mosaic disease resistance in an F<sub>2</sub> population derived from the cross between \"Nongke 1-6\" (H. vulgare) and \"Zaoshu 3\" (H. vulgare). This QTL, originating from bulbous barley (Hordeum bulbosum), demonstrated stability and was further validated in another F<sub>2</sub> population derived from the cross between \"Nongke 2-6\" (H. vulgare) and \"Supi 1\" (H. vulgare). QRym.ZN1-7H accounted for 10.61%-19.34% of the phenotypic variance. The QTL was further fine mapped to the 14- to 39-Mb interval on barley chromosome 7H. Transcriptome analysis identified 53 and 35 differentially expressed genes in roots and leaves (at QRym.ZN1-7H locus), respectively, with nine genes differentially expressing in both tissues. HORVU.MOREX.r3.7HG0650990, a member of the disease resistance protein family (NBS-LRR class), is the most likely candidate gene for QRym.ZN1-7H. Enrichment analysis indicated that QRym.ZN1-7H may be involved in signal transduction in plant innate immune response. This study laid a foundation for barley disease resistance breeding.</p>","PeriodicalId":49002,"journal":{"name":"Plant Genome","volume":"18 1","pages":"e20557"},"PeriodicalIF":3.9000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11726411/pdf/","citationCount":"0","resultStr":"{\"title\":\"Identification and characterization of a novel QTL for barley yellow mosaic disease resistance from bulbous barley.\",\"authors\":\"Yi Hong, Hui Zhou, Mengna Zhang, Yuhang Zhang, Juan Zhu, Chao Lv, Baojian Guo, Feifei Wang, Qingliang Li, Jie Sun, Rugen Xu\",\"doi\":\"10.1002/tpg2.20557\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Winter barley (Hordeum vulgare) production areas in the middle and lower reaches of the Yangtze River are severely threatened by barley yellow mosaic disease, which is caused by Barley yellow mosaic virus and Barley mild mosaic virus. Improving barley disease resistance in breeding programs requires knowledge of genetic loci in germplasm resources. In this study, bulked segregant analysis (BSA) identified a novel major quantitative trait loci (QTL) QRym.ZN1-7H for barley yellow mosaic disease resistance in an F<sub>2</sub> population derived from the cross between \\\"Nongke 1-6\\\" (H. vulgare) and \\\"Zaoshu 3\\\" (H. vulgare). This QTL, originating from bulbous barley (Hordeum bulbosum), demonstrated stability and was further validated in another F<sub>2</sub> population derived from the cross between \\\"Nongke 2-6\\\" (H. vulgare) and \\\"Supi 1\\\" (H. vulgare). QRym.ZN1-7H accounted for 10.61%-19.34% of the phenotypic variance. The QTL was further fine mapped to the 14- to 39-Mb interval on barley chromosome 7H. Transcriptome analysis identified 53 and 35 differentially expressed genes in roots and leaves (at QRym.ZN1-7H locus), respectively, with nine genes differentially expressing in both tissues. HORVU.MOREX.r3.7HG0650990, a member of the disease resistance protein family (NBS-LRR class), is the most likely candidate gene for QRym.ZN1-7H. Enrichment analysis indicated that QRym.ZN1-7H may be involved in signal transduction in plant innate immune response. This study laid a foundation for barley disease resistance breeding.</p>\",\"PeriodicalId\":49002,\"journal\":{\"name\":\"Plant Genome\",\"volume\":\"18 1\",\"pages\":\"e20557\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2025-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11726411/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Genome\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1002/tpg2.20557\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Genome","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1002/tpg2.20557","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0

摘要

大麦黄花叶病是由大麦黄花叶病病毒和大麦轻度花叶病病毒引起的,是长江中下游冬大麦产区的严重病害。在育种计划中提高大麦抗病能力需要对种质资源中的遗传位点有所了解。本研究通过本体分离分析(BSA)鉴定出一个新的主要数量性状位点(QRym)。“农科1-6”与“枣树3号”杂交F2群体大麦黄花叶病抗性基因ZN1-7H。该QTL来源于球茎大麦(Hordeum bulbosum),在“农科2-6”(H. vulgare)和“苏皮1”(H. vulgare)杂交的F2群体中得到了进一步的验证。QRym。ZN1-7H占表型方差的10.61% ~ 19.34%。进一步将QTL精细定位到大麦7H染色体14 ~ 39mb区间。转录组分析鉴定出53个和35个差异表达基因在QRym的根和叶中。ZN1-7H位点),有9个基因在两种组织中差异表达。HORVU.MOREX.r3.7HG0650990是抗病蛋白家族(NBS-LRR类)的成员,是QRym.ZN1-7H最有可能的候选基因。富集分析表明QRym。ZN1-7H可能参与植物先天免疫应答的信号转导。本研究为大麦抗病育种奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification and characterization of a novel QTL for barley yellow mosaic disease resistance from bulbous barley.

Winter barley (Hordeum vulgare) production areas in the middle and lower reaches of the Yangtze River are severely threatened by barley yellow mosaic disease, which is caused by Barley yellow mosaic virus and Barley mild mosaic virus. Improving barley disease resistance in breeding programs requires knowledge of genetic loci in germplasm resources. In this study, bulked segregant analysis (BSA) identified a novel major quantitative trait loci (QTL) QRym.ZN1-7H for barley yellow mosaic disease resistance in an F2 population derived from the cross between "Nongke 1-6" (H. vulgare) and "Zaoshu 3" (H. vulgare). This QTL, originating from bulbous barley (Hordeum bulbosum), demonstrated stability and was further validated in another F2 population derived from the cross between "Nongke 2-6" (H. vulgare) and "Supi 1" (H. vulgare). QRym.ZN1-7H accounted for 10.61%-19.34% of the phenotypic variance. The QTL was further fine mapped to the 14- to 39-Mb interval on barley chromosome 7H. Transcriptome analysis identified 53 and 35 differentially expressed genes in roots and leaves (at QRym.ZN1-7H locus), respectively, with nine genes differentially expressing in both tissues. HORVU.MOREX.r3.7HG0650990, a member of the disease resistance protein family (NBS-LRR class), is the most likely candidate gene for QRym.ZN1-7H. Enrichment analysis indicated that QRym.ZN1-7H may be involved in signal transduction in plant innate immune response. This study laid a foundation for barley disease resistance breeding.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Plant Genome
Plant Genome PLANT SCIENCES-GENETICS & HEREDITY
CiteScore
6.00
自引率
4.80%
发文量
93
审稿时长
>12 weeks
期刊介绍: The Plant Genome publishes original research investigating all aspects of plant genomics. Technical breakthroughs reporting improvements in the efficiency and speed of acquiring and interpreting plant genomics data are welcome. The editorial board gives preference to novel reports that use innovative genomic applications that advance our understanding of plant biology that may have applications to crop improvement. The journal also publishes invited review articles and perspectives that offer insight and commentary on recent advances in genomics and their potential for agronomic improvement.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信