ADAR同工型的编辑特异性。

4区 生物学 Q3 Biochemistry, Genetics and Molecular Biology
Methods in enzymology Pub Date : 2025-01-01 Epub Date: 2024-12-04 DOI:10.1016/bs.mie.2024.11.021
Cornelia Vesely, Michael F Jantsch
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引用次数: 0

摘要

作用于RNA (ADARs)酶的腺苷到肌苷脱氨酶存在于所有后生动物中。它们的序列和蛋白质组织是保守的,但也表现出明显的差异。此外,ADAR基因的数量因生物而异,从苍蝇的1个到哺乳动物的3个不等。ADARs的不同同工异构体及其特定作用决定了A-to-I RNA编辑、调控和这些酶的多功能性的复杂性。了解不同的同工异构体特异性功能和靶标将有助于更深入地了解受ADAR影响的各种生物过程,无论是通过ADAR编辑dsrna还是与rna和蛋白质的相互作用。详细鉴定和指定特异性靶异构体是我们了解ADAR异构体之间功能差异的关键一步,并将帮助我们了解它们对健康和疾病的个体影响。本章深入探讨了ADAR异构体的独特特征和功能含义。我们描述了编辑自由细胞中的异位过表达,并使用RNA免疫沉淀与测序相结合来确定与RNA及其编辑位点的异构体特异性相互作用。此外,我们讨论了在其他修改的背景下,不同adar在编辑检测方面的新挑战,并为确定“真正编辑”的潜在更好方法提供了想法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Editing specificity of ADAR isoforms.

Adenosine to inosine deaminases acting on RNA (ADARs) enzymes are found in all metazoa. Their sequence and protein organization is conserved but also shows distinct differences. Moreover, the number of ADAR genes differs between organisms, ranging from one in flies to three in mammals. The distinct isoforms of ADARs and their specific roles determine the complexity of A-to-I RNA editing, its regulation and the versatility of these enzymes. Understanding the different isoform-specific functions and targets will provide a deeper understanding of the diverse biological processes influenced by ADARs, either through ADAR editing of dsRNAs or the interaction with RNAs and proteins. The detailed identification and assigning of isoform-specific targets is a crucial step towards our understanding of functional differences amongst ADAR isoforms and will help us to understand their individual implications for health and disease. This chapter delves into unique characteristics and functional implications of ADAR isoforms. We describe the ectopic overexpression in editing free cells and the use of RNA immunoprecipitation coupled with sequencing to determine isoform-specific interactions with RNAs and their editing sites. Additionally, we discuss new challenges in editing detection by different ADARs in the context of other modifications and provide ideas for potentially better methods to determine the "true editome".

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来源期刊
Methods in enzymology
Methods in enzymology 生物-生化研究方法
CiteScore
2.90
自引率
0.00%
发文量
308
审稿时长
3-6 weeks
期刊介绍: The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 500 volumes the series contains much material still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research and genetics-just to name a few. Five of the 2013 Nobel Laureates have edited or contributed to volumes of MIE.
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