不同灭活剂制备的肺炎支原体和猪支原体疫苗的灭活性和免疫原性的比较研究。

Yanna Wei, Jia Wang, Li Wang, Beibei Liu, Ting Yuan, Yanfei Yu, Guoqing Shao, Zhixin Feng, Thandeka Ntokozo Khoza, Qiyan Xiong
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引用次数: 0

摘要

本研究旨在探讨低肺炎支原体和低喉支原体灭活疫苗的最佳灭活剂和灭活剂条件。用甲醛、硫柳汞、β-丙内酯(BPL)和二亚胺(BEI)进行支原体灭活,并进行比较。结果表明,在37℃条件下,0.01%甲醛孵育24 h或0.02%甲醛孵育12 h, 0.0008%硫脲孵育12 h, 0.02% BPL孵育24 h或0.1% BPL孵育12 h, 0.004% BEI孵育24 h或0.5% BEI孵育12 h, 37℃条件下,肺炎支原体完全失活。用0.01%甲醛或0.02%甲醛在37℃下孵育24小时或12小时,用0.004%硫柳汞在37℃下孵育24小时或0.02%硫柳汞在37℃下孵育12小时,用0.1% BPL在4℃下孵育12小时,或用0.004% BEI在37℃下孵育24小时或0.5% BEI在37℃下孵育12小时。接下来,通过免疫BALB/c小鼠来评估灭活后支原体的免疫原性。用高剂量(每剂量106个颜色变化单位[CCU])的猪肺炎支原体和猪肺支原体灭活疫苗免疫小鼠可导致血清IgG抗体水平升高。用甲醛灭活的猪肺炎支原体疫苗诱导的抗体滴度显著高于用其他灭活剂灭活的疫苗,而用BEI灭活的猪肺炎支原体疫苗诱导的抗体滴度显著高于硫柳汞灭活疫苗。然而,在低剂量支原体免疫小鼠(每剂量104 CCU)中,只有甲醛和BEI灭活的猪肺炎支原体疫苗和甲醛、BPL和BEI灭活的猪支原体疫苗导致血清IgG抗体水平显著升高。其中,甲醛灭活疫苗组的抗体水平高于其他灭活疫苗组。本研究为大规模生产肺炎支原体和缩支原体灭活疫苗提供了可靠的灭活依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative study on the inactivation and immunogenicity of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis vaccines prepared using different inactivants.

The present study aimed to investigate the optimal inactivants and inactivation conditions for preparing inactivated vaccines of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis. Mycoplasma inactivation was performed using formaldehyde, thimerosal, β-propiolactone (BPL), and binary ethylenimine (BEI) and compared. The results showed that M. hyopneumoniae was completely inactivated when incubated with 0.01 % formaldehyde for 24 h or 0.02 % formaldehyde for 12 h at 37 °C, with 0.0008 % thimerosal for 12 h at 37 °C, with 0.02 % BPL for 24 h or 0.1 % BPL for 12 h at 4 °C, or with 0.004 % BEI for 24 h or 0.5 % BEI for 12 h at 37 °C. M. hyorhinis was completely inactivated when incubated with 0.01 % formaldehyde for 24 h or 0.02 % formaldehyde for 12 h at 37 °C, with 0.004 % thimerosal for 24 h or 0.02 % thimerosal for 12 h at 37 °C, with 0.1 % BPL for 12 h at 4 °C, or with 0.004 % BEI for 24 h or 0.5 % BEI for 12 h at 37 °C. Next, the immunogenicity of the mycoplasmas after inactivation was evaluated by immunizing BALB/c mice. Immunization of mice with a high dose (106 color-changing units [CCU] per dose) of M. hyopneumoniae and M. hyorhinis vaccines inactivated with all inactivants led to high levels of serum IgG antibodies. M. hyopneumoniae vaccines inactivated with formaldehyde induced significantly higher titers of antibodies than vaccines inactivated with other inactivants, whereas M. hyorhinis vaccines inactivated with BEI induced significantly higher titers of antibodies than vaccines inactivated with thimerosal. However, in mice immunized with a low dose of mycoplasmas (104 CCU per dose), only M. hyopneumoniae vaccines inactivated with formaldehyde and BEI and M. hyorhinis vaccines inactivated with formaldehyde, BPL, and BEI led to significant levels of serum IgG antibodies. Among these groups, the antibody levels in the formaldehyde-inactivated vaccine group were higher than those in the other inactivant groups. This study provides a reliable basis for inactivation during large-scale production of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis inactivated vaccines.

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