转录组学揭示了产后酮症奶牛肌肉组织中circRNA的调控机制。

IF 3 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Tao Tang , Jing Zhou , Meigui Wang , Siqi Xia , Wenqiang Sun , Xianbo Jia , Jie Wang , Songjia Lai
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引用次数: 0

摘要

奶牛从妊娠晚期到泌乳早期的过渡阶段会发生显著的代谢变化,以应对能量代谢方面的挑战。肌肉组织作为奶牛体内最大的能量代谢组织,在能量代谢中起着至关重要的作用。此外,环状rna (circRNAs)已被证明在各种生物事件中发挥关键作用。然而,在酮症奶牛的肌肉组织中,环状rna介导的能量代谢和肌肉细胞的调节机制尚不清楚。在这里,我们在受酮症影响的奶牛的肌肉组织中鉴定了总共5103个环状rna。其中,与健康奶牛相比,57个环状rna在酮症奶牛的肌肉组织中差异表达,其中39个上调,18个下调。基于circRNAs源基因的功能富集分析表明,circ-30,628与碳代谢密切相关,circ-CoQ2与线粒体能量代谢相关。鉴于circRNAs对mirna的海绵效应,我们进一步预测了circ-30,628和circ-CoQ2的下游mirna和mrna的网络关系,发现它们的下游靶基因参与了MAPK、Wnt、FoxO、自噬等信号通路,与肌肉细胞的增殖、分化、能量代谢、氧化应激、线粒体功能等相关。综上所述,这些发现为了解circrna在奶牛酮症肌肉组织中调节能量代谢的功能,从而减少酮体的积累,预防奶牛酮症的发生提供了理论基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Transcriptomics reveals the regulatory mechanisms of circRNA in the muscle tissue of cows with ketosis postpartum
The transition period from late pregnancy to early lactation in dairy cows involves significant metabolic changes to cope with the challenges related to energy metabolism. Muscle tissue, as the largest energy-metabolizing tissue in dairy cows, plays a crucial role in energy metabolism. Furthermore, circular RNAs (circRNAs) have been shown to play key roles in various biological events. However, the regulatory mechanisms of energy metabolism and muscle cells mediated by circRNAs in the muscle tissue of ketotic dairy cows remain unclear. Here, we identified a total of 5103 circRNAs in the muscle tissue of ketosis-affected cows. Among these, compared to healthy cows, 57 circRNAs were differentially expressed in the muscle tissue of ketosis-affected cows, with 39 upregulated and 18 downregulated. Functional enrichment analysis based on the source genes of circRNAs indicated that circ-30,628 is closely related to carbon metabolism, and circ-CoQ2 is associated with mitochondrial energy metabolism. Given the sponge effect of circRNAs on miRNAs, we further predicted the network relationships of downstream miRNAs and mRNAs of circ-30,628 and circ-CoQ2, and found that their downstream target genes are involved in signaling pathways such as MAPK, Wnt, FoxO, and autophagy, which is associated with the proliferation, differentiation, energy metabolism, oxidative stress, and mitochondrial function of muscle cells. In summary, these findings provide a theoretical basis for understanding the functions of circRNAs regulating energy metabolism in the muscle tissue of ketosis-affected cows, thereby reducing the accumulation of ketone bodies to prevent the occurrence of ketosis in dairy cows.
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来源期刊
Genomics
Genomics 生物-生物工程与应用微生物
CiteScore
9.60
自引率
2.30%
发文量
260
审稿时长
60 days
期刊介绍: Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.
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