具有重要生态和经济意义的兔鱼（Siganus guttatus）和兔鱼（Siganus oramin）的染色体规模基因组。

IF 3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2025-01-23 DOI:10.1016/j.ygeno.2025.111007

Lin Xian , Sunil Kumar Sahu , Xiaolin Huang , Chao Li , Yu Liang , Yan Ou-Yang , Huayang Guo , Bo Liu , Kecheng Zhu , Baosuo Liu , Nan Zhang , Tengfei Zhu , Qiye Li , Dianchang Zhang

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引用次数: 0

摘要

guttatus Siganus和oramin Siganus是东太平洋沿岸自然分布的两个主要物种，具有相当的生态和经济价值。在这里，我们提出了这两个Siganus物种的染色体水平基因组的构建和比较分析。采用杂交组装策略，对S. guttatus和S. oramin的PacBio、Illumina和Hi-C reads进行了分割和独立组装，得到了染色体水平的基因组。两个基因组的组装大小（N50大小，BUSCO完整性）分别为642.4 M（25.76 M, 98.26 %）和502.8 M(16.98 M, 98.8 %)，具有较高的连续性和完整性。这项研究标志着第一次成功组装了Siganus物种的染色体水平基因组，以及通过比较基因组学分析对其饮食习惯和栖息地的初步探索。这些发现为比较基因组学和分子进化研究提供了重要的资源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Chromosome-scale genomes of ecologically and economically important rabbitfish Siganus guttatus and Siganus oramin

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Chromosome-scale genomes of ecologically and economically important rabbitfish Siganus guttatus and Siganus oramin

Siganus guttatus and Siganus oramin are two major species that are naturally distributed along the Eastern Pacific coast and possess considerable ecological and economic value. Here, we present the construction and comparative analysis of the chromosome-level genomes of these two Siganus species. Employing a hybrid assembly strategy, we partitioned and independently assembled the PacBio, Illumina and Hi-C reads of S. guttatus and S. oramin, resulting in chromosome-level genomes. The assembly sizes (N50 size, BUSCO completeness) of the two genomes were 642.4 M (25.76 M, 98.26 %) and 502.8 M (16.98 M, 98.8 %) for S. guttatus and S. oramin, respectively, exhibiting high contiguity and integrity. This study marks the first successful assembly of chromosome-level genomes in Siganus species, along with an initial exploration of their dietary habits and habitats through comparative genomics analysis. These findings offer essential resources for comparative genomics and molecular evolution research.

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.