Aage Kristian Olsen Alstrup, Mette Simonsen, Kim Vang Hansen, Caroline C Real
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引用次数: 0
摘要
背景：在临床前研究中，麻醉可以显著影响正电子发射断层扫描（PET）神经成像。因此，了解这些影响对于准确解释结果至关重要。在本实验中，我们研究了[18F]标记的葡萄糖类似物氟脱氧葡萄糖（[18F]FDG）在被两种常用的啮齿动物麻醉剂麻醉的大鼠大脑中摄取的影响：异氟醚（一种吸入性麻醉剂）和Hypnorm-Dormicum（一种联合注射麻醉剂）。材料和方法：雌性成年sd大鼠随机分为异氟醚组和低睡眠眠酮组。对大鼠进行动态[18F]FDG PET扫描。全脑[18F]FDG标准摄取值（SUV）及基于脑体素的分析。结果：动态[18F]FDG数据显示，异氟醚组成像40 min后脑SUV降低38%（2.085±0.3563 vs. 3.369±0.5577,p = 0.0008）。在两组间基于体素的分析中，这些图谱与SUV数据相结合，揭示了异氟醚组[18F]FDG摄取的减少，主要是在皮质区域，在中脑和小脑中观察到额外的少量增加。讨论和结论：观察到的脑内[18F]FDG摄取的差异可能归因于代谢活动的变化。这些结果强调了在未来研究中仔细考虑麻醉选择及其对神经影像学结果的影响的必要性。
Metabolic Differences in Neuroimaging with [18F]FDG in Rats Under Isoflurane and Hypnorm-Dormicum.
Background: Anesthesia can significantly impact positron emission tomography (PET) neuroimaging in preclinical studies. Therefore, understanding these effects is crucial for accurate interpretation of the results. In this experiment, we investigate the effect of [18F]-labeled glucose analog fluorodeoxyglucose ([18F]FDG) uptake in the brains of rats anesthetized with two commonly used anesthetics for rodents: isoflurane, an inhalation anesthetic, and Hypnorm-Dormicum, a combination injection anesthetic.
Materials and methods: Female adult Sprague Dawley rats were randomly assigned to one of two anesthesia groups: isoflurane or Hypnorm-Dormicum. The rats were submitted to dynamic [18F]FDG PET scan. The whole brain [18F]FDG standard uptake value (SUV) and the brain voxel-based analysis were performed.
Results: The dynamic [18F]FDG data revealed that the brain SUV was 38% lower in the isoflurane group after 40 min of image (2.085 ± 0.3563 vs. 3.369 ± 0.5577, p = 0.0008). In voxel-based analysis between groups, the maps collaborate with SUV data, revealing a reduction in [18F]FDG uptake in the isoflurane group, primarily in the cortical regions, with additional small increases observed in the midbrain and cerebellum.
Discussion and conclusions: The observed differences in [18F]FDG uptake in the brain may be attributed to variations in metabolic activity. These results underscore the necessity for careful consideration of anesthetic choice and its impact on neuroimaging outcomes in future research.
TomographyMedicine-Radiology, Nuclear Medicine and Imaging
CiteScore
2.70
自引率
10.50%
发文量
222
期刊介绍：
TomographyTM publishes basic (technical and pre-clinical) and clinical scientific articles which involve the advancement of imaging technologies. Tomography encompasses studies that use single or multiple imaging modalities including for example CT, US, PET, SPECT, MR and hyperpolarization technologies, as well as optical modalities (i.e. bioluminescence, photoacoustic, endomicroscopy, fiber optic imaging and optical computed tomography) in basic sciences, engineering, preclinical and clinical medicine.
Tomography also welcomes studies involving exploration and refinement of contrast mechanisms and image-derived metrics within and across modalities toward the development of novel imaging probes for image-based feedback and intervention. The use of imaging in biology and medicine provides unparalleled opportunities to noninvasively interrogate tissues to obtain real-time dynamic and quantitative information required for diagnosis and response to interventions and to follow evolving pathological conditions. As multi-modal studies and the complexities of imaging technologies themselves are ever increasing to provide advanced information to scientists and clinicians.
Tomography provides a unique publication venue allowing investigators the opportunity to more precisely communicate integrated findings related to the diverse and heterogeneous features associated with underlying anatomical, physiological, functional, metabolic and molecular genetic activities of normal and diseased tissue. Thus Tomography publishes peer-reviewed articles which involve the broad use of imaging of any tissue and disease type including both preclinical and clinical investigations. In addition, hardware/software along with chemical and molecular probe advances are welcome as they are deemed to significantly contribute towards the long-term goal of improving the overall impact of imaging on scientific and clinical discovery.
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