不同诱导剂作用下神经干细胞衍生伪神经细胞凋亡水平的评估。

IF 1.5 Q4 CELL BIOLOGY
American journal of stem cells Pub Date : 2024-12-15 eCollection Date: 2024-01-01 DOI:10.62347/BPTG6174
Adele Naghshbandieh, Atefe Naghshbandieh, Elahe Barfi, Leila Abkhooie
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引用次数: 0

摘要

神经系统的发育和维持是由一种被称为细胞凋亡的细胞死亡机制所控制的。神经元的存活和功能在很大程度上取决于从神经干细胞分化产生的伪神经元细胞的死亡程度。不同的诱导剂可影响这些细胞的死亡程度:激素、药物、生长因子等。开发神经退行性疾病的创新疗法取决于这些诱导剂如何影响分化的伪神经元细胞的死亡率的知识。利用流式细胞术、Western blotting和荧光显微镜等技术,对许多伪神经元细胞的死亡程度进行了评估。流式细胞术通过测量细胞大小、粒度和DNA含量来产生死细胞计数。荧光显微镜使用荧光染料或抗体观察死细胞,而Western blotting检测caspases和Bcl-2家族蛋白。本文就不同诱导剂作用下神经干细胞分化的伪神经细胞死亡的研究现状作一综述。通过研究这些诱导剂如何影响死亡,本综述旨在为指导下一步的研究和支持神经退行性疾病的治疗计划提供信息。着眼于像维甲酸、斯来吉兰、细胞因子、丙戊酸和小化合物这样的诱导剂,我们检查了研究以评估死亡率。这些发现为指导这些细胞死亡的分子过程提供了重要的新视角。对于不同因素如何影响导致死亡的分子过程仍然完全缺乏了解,因此了解这些过程可以有助于治疗神经退行性疾病的新治疗方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment of the level of apoptosis in differentiated pseudo-neuronal cells derived from neural stem cells under the influence of various inducers.

Development and maintenance of the nervous system are governed by a scheduled cell death mechanism known as apoptosis. Very much how neurons survive and function depends on the degree of death in differentiating pseudo-neuronal cells produced from neural stem cells. Different inducers can affect the degree of death in these cells: hormones, medicines, growth factors, and others. Developing inventive therapies for neurodegenerative illnesses depends on a knowledge of how these inducers impact mortality in differentiated pseudo-neuronal cells. Using flow cytometry, Western blotting, and fluorescence microscopy among other techniques, the degree of death in many pseudo-neuronal cells is evaluated. Flow cytometry generates dead cell counts from measurements of cell size, granularity, and DNA content. Whereas fluorescence microscopy visualizes dead cells using fluorescent dyes or antibodies, Western blotting detects caspases and Bcl-2 family proteins. This review attempts to offer a thorough investigation of present studies on death in differentiated pseudo-neuronal cells produced from neural stem cells under the effect of different inducers. Through investigating how these inducers influence death, the review aims to provide information that might direct the next studies and support treatment plans for neurodegenerative diseases. With an eye toward inducers like retinoic acid, selegiline, cytokines, valproic acid, and small compounds, we examined research to evaluate death rates. The findings offer important new perspectives on the molecular processes guiding death in these cells. There is still a complete lack of understanding of how different factors affect the molecular processes that lead to death, so understanding these processes can contribute to new therapeutic approaches to treat neurodegenerative diseases.

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