气真皮蛋白D作为坏死性小肠结肠炎生物标志物的诊断准确性:一项单中心诊断试验研究

IF 1.5 4区 医学 Q2 PEDIATRICS
Translational pediatrics Pub Date : 2024-12-31 Epub Date: 2024-12-27 DOI:10.21037/tp-24-326
Shan He, Xiao-Qian Zhang, Meng-Ran Fu, Hong-Ying Mi
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引用次数: 0

摘要

背景:坏死性小肠结肠炎(NEC)是一种主要影响早产儿的破坏性胃肠道疾病,气皮蛋白D (GSDMD)因其在NEC发病过程中称为焦亡的炎症过程中的关键作用而成为人们感兴趣的分子。本研究的目的是研究GSDMD和白细胞介素-1β (IL-1β)作为NEC早期诊断生物标志物的潜力。方法:我们对2023年12月至2024年6月期间入住新生儿重症监护病房(NICU)的207名有NEC临床症状的婴儿进行了调查。在排除先天性胃肠疾病和其他因素后,180名婴儿被纳入研究。其中59例经Bell II期标准确诊为NEC, 56例经倾向评分匹配(PSM)选择匹配对照。采用定量聚合酶链反应(qPCR)和酶联免疫吸附试验(ELISA)检测血清中GSDMD的表达和IL-1β的水平。结果:与对照组相比,NEC患者GSDMD(18.46±8.58)和IL-1β(9.05±3.42)水平显著升高。受试者工作特征(ROC)曲线分析表明,GSDMD对NEC的预测价值高于IL-1β (AUC 0.77),曲线下面积为0.83。临床症状如疲劳、腹胀和肠音减少在NEC患者中更为常见。实验室结果显示,NEC患者的中性粒细胞和红细胞计数较低,血小板计数较高,c反应蛋白(CRP)和降钙素原(PCT)水平升高。结论:GSDMD和IL-1β可以作为NEC早期诊断的有价值的生物标志物,为了解该疾病的发病机制和促进早期发现和干预策略的改进提供了依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Diagnostic accuracy of gasdermin D as a biomarker for necrotizing enterocolitis: a single-center diagnostic test study.

Background: Necrotizing enterocolitis (NEC) is a devastating gastrointestinal condition mainly affecting premature infants, and gasdermin D (GSDMD) has emerged as a molecule of interest due to its pivotal role in the inflammatory process called pyroptosis in NEC pathogenesis. The aim of this study is to examine the potential of GSDMD and interleukin-1β (IL-1β) as early diagnostic biomarkers for NEC.

Methods: We examined 207 infants with clinical symptoms of NEC admitted to our neonatal intensive care unit (NICU) between December 2023 and June 2024. After excluding those with congenital gastrointestinal diseases and other factors, 180 infants were included in the study. Among these, 59 were confirmed to have NEC according to Bell Stage II criteria, and 56 matched controls were selected through propensity score matching (PSM). Blood samples were analyzed for GSDMD expression using quantitative polymerase chain reaction (qPCR) and for IL-1β levels using enzyme-linked immunosorbent assay (ELISA).

Results: Patients with NEC exhibited significantly elevated levels of GSDMD (18.46±8.58) and IL-1β (9.05±3.42) compared to controls. Receiver operating characteristic (ROC) curve analysis indicated that GSDMD had a higher predictive value for NEC with an area under the curve (AUC) of 0.83 than IL-1β (AUC 0.77). Clinical symptoms such as fatigue, abdominal distention, and reduced bowel sounds were significantly more common in patients with NEC. Laboratory results showed lower neutrophil and red blood cell counts, higher platelet counts, and increased levels of C-reactive protein (CRP) and procalcitonin (PCT) in patients with NEC.

Conclusions: GSDMD and IL-1β can serve as valuable biomarkers for the early diagnosis of NEC, providing insights into the disease's pathogenesis and facilitating improved strategies for early detection and intervention.

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来源期刊
Translational pediatrics
Translational pediatrics Medicine-Pediatrics, Perinatology and Child Health
CiteScore
4.50
自引率
5.00%
发文量
108
期刊介绍: Information not localized
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