{"title":"年龄、采收地点和体重指数对基质血管部分细胞组成的影响。","authors":"Zi-Han Yang, Tian-Yu Zhang, Fang-Zhou Chen, Yun Xie, Poh-Ching Tan, Qing-Feng Li, Shuang-Bai Zhou","doi":"10.1097/PRS.0000000000011970","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The stromal vascular fraction (SVF) of adipose tissue has now been widely used in plastic surgeries, clinical trials and therapies. However, the cell composition of SVF undergoes dynamic changes during aging and obesity, which may influence the efficacy of the SVF. This study analyzed the effects of age, harvest site and body mass index on the cell composition of the SVF.</p><p><strong>Methods: </strong>A study was performed on 162 patients. The age ranged from 13 to 88 years old, with body mass index (BMI) <25kg/m2 or ≥ 25kg/m2. Fat tissue was harvested from the abdominal region, hips, thighs or arms, and the SVF was isolated for flow cytometry.</p><p><strong>Results: </strong>Our results demonstrated that the declines of adipose derived stem cells (ADSCs) and endothelial cells (ECs) first occurred in donors aged 40-49 years old. Subsequently, the percentages of endothelial progenitor cells (EPCs), preadipocytes, and M2 macrophages started to decrease in donors aged 50-69 years old. Lower percentages of ECs and M2 macrophages were found in donors with higher BMI.</p><p><strong>Conclusions: </strong>This study demonstrated that the percentages of ADSCs and ECs of SVF, decreased significantly starting from donors over age 40, followed by changes in other cell subpopulations. Furthermore, donors with BMI ≥ 25kg/m2 had lower percentages of ECs and M2 macrophages.</p>","PeriodicalId":20128,"journal":{"name":"Plastic and reconstructive surgery","volume":" ","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of age, harvest site and body mass index on the cell composition of the stromal vascular fraction.\",\"authors\":\"Zi-Han Yang, Tian-Yu Zhang, Fang-Zhou Chen, Yun Xie, Poh-Ching Tan, Qing-Feng Li, Shuang-Bai Zhou\",\"doi\":\"10.1097/PRS.0000000000011970\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The stromal vascular fraction (SVF) of adipose tissue has now been widely used in plastic surgeries, clinical trials and therapies. However, the cell composition of SVF undergoes dynamic changes during aging and obesity, which may influence the efficacy of the SVF. This study analyzed the effects of age, harvest site and body mass index on the cell composition of the SVF.</p><p><strong>Methods: </strong>A study was performed on 162 patients. The age ranged from 13 to 88 years old, with body mass index (BMI) <25kg/m2 or ≥ 25kg/m2. Fat tissue was harvested from the abdominal region, hips, thighs or arms, and the SVF was isolated for flow cytometry.</p><p><strong>Results: </strong>Our results demonstrated that the declines of adipose derived stem cells (ADSCs) and endothelial cells (ECs) first occurred in donors aged 40-49 years old. Subsequently, the percentages of endothelial progenitor cells (EPCs), preadipocytes, and M2 macrophages started to decrease in donors aged 50-69 years old. Lower percentages of ECs and M2 macrophages were found in donors with higher BMI.</p><p><strong>Conclusions: </strong>This study demonstrated that the percentages of ADSCs and ECs of SVF, decreased significantly starting from donors over age 40, followed by changes in other cell subpopulations. Furthermore, donors with BMI ≥ 25kg/m2 had lower percentages of ECs and M2 macrophages.</p>\",\"PeriodicalId\":20128,\"journal\":{\"name\":\"Plastic and reconstructive surgery\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2025-01-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plastic and reconstructive surgery\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1097/PRS.0000000000011970\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"SURGERY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plastic and reconstructive surgery","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1097/PRS.0000000000011970","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SURGERY","Score":null,"Total":0}
Effect of age, harvest site and body mass index on the cell composition of the stromal vascular fraction.
Background: The stromal vascular fraction (SVF) of adipose tissue has now been widely used in plastic surgeries, clinical trials and therapies. However, the cell composition of SVF undergoes dynamic changes during aging and obesity, which may influence the efficacy of the SVF. This study analyzed the effects of age, harvest site and body mass index on the cell composition of the SVF.
Methods: A study was performed on 162 patients. The age ranged from 13 to 88 years old, with body mass index (BMI) <25kg/m2 or ≥ 25kg/m2. Fat tissue was harvested from the abdominal region, hips, thighs or arms, and the SVF was isolated for flow cytometry.
Results: Our results demonstrated that the declines of adipose derived stem cells (ADSCs) and endothelial cells (ECs) first occurred in donors aged 40-49 years old. Subsequently, the percentages of endothelial progenitor cells (EPCs), preadipocytes, and M2 macrophages started to decrease in donors aged 50-69 years old. Lower percentages of ECs and M2 macrophages were found in donors with higher BMI.
Conclusions: This study demonstrated that the percentages of ADSCs and ECs of SVF, decreased significantly starting from donors over age 40, followed by changes in other cell subpopulations. Furthermore, donors with BMI ≥ 25kg/m2 had lower percentages of ECs and M2 macrophages.
期刊介绍：
For more than 70 years Plastic and Reconstructive Surgery® has been the one consistently excellent reference for every specialist who uses plastic surgery techniques or works in conjunction with a plastic surgeon. Plastic and Reconstructive Surgery® , the official journal of the American Society of Plastic Surgeons, is a benefit of Society membership, and is also available on a subscription basis.
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