海绵状绒毛海绵诱导A549人肺癌细胞信号转导及转录-3激活因子的抑制

Q2 Medicine
Journal of Experimental Pharmacology Pub Date : 2025-01-11 eCollection Date: 2025-01-01 DOI:10.2147/JEP.S494158
Yuni Elsa Hadisaputri, Annida Adha Nurhaniefah, Mutakin Mutakin, Rini Hendriani, Andri Rezano, Iyan Sopyan, Yusnaini Yusnaini, Yonathan Asikin, Rizky Abdulah
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引用次数: 0

摘要

肺癌是公认的高致死率疾病,需要快速准确的解决方案。先前的研究表明,含麦角-22- en3- one和麦角-7-en3-ol的aerizusa (C. aerizusa) Callyspongia提取物对A549肺癌细胞具有细胞毒作用,IC50值为9.38 μg/mL。然而,提取物对Het-1A食管上皮细胞没有细胞毒性。几篇综述也证实了与caspase依赖性信号通路相关的促凋亡分子的上调和抗凋亡分子的抑制。目的:本研究的目的是扩大对蛇麻提取物对A549肺癌的作用的认识,研究其对多种信号通路、恶性、迁移和侵袭的影响。材料和方法:PCR检测mRNA表达,靶向PTEN、Akt、mTOR、STAT-3、IL-6、VEGF、HIF1α。Western Blot检测PTEN、p-Akt、Akt、p-mTOR、p-STAT-3蛋白表达。采用创面愈合和侵袭实验,检测爱丽草提取物处理后A549细胞的迁移和侵袭能力。结果:mRNA表达分析显示,给药24 h后,Akt和m-TOR表达升高,PTEN和STAT-3表达降低。在蛋白水平上,p-Akt、Akt、p-mTOR和p-STAT-3在24小时治疗期间下调,而PTEN升高。伤口愈合和侵袭实验结果显示,A549细胞在24小时的处理后,伤口愈合和侵袭能力减弱。IL-6和HIF-1α mRNA在24小时内表达水平下降,VEGF mRNA与未处理细胞相比略有下降。结论:综上所述,海绵C. aerizusa提取物中的麦角甾体对A549肺癌细胞的恶性、迁移和侵袭能力有显著的降低作用。这些结果表明它们在未来的抗癌治疗中具有抗血管生成的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Suppression of Signal Transducer and Activator of Transcription-3 in A549 human Lung Carcinoma Cells Induced by Marine Sponge Callyspongia aerizusa.

Introduction: Lung cancer is recognized as a highly lethal disease, demanding swift and accurate solutions. Previous analysis showed the cytotoxic impact of Callyspongia aerizusa (C. aerizusa) extract containing ergost-22-en-3-one and ergost-7-en3-ol against A549 lung cancer cells, with an IC50 value of 9.38 μg/mL. However, the extract did not have cytotoxicity towards Het-1A esophagus epithelial cells. Several reviews also validated the upregulation of pro-apoptotic molecules and the inhibition of anti-apoptotic molecules linked to the caspase-dependent signaling pathway.

Purpose: The objective of this research was to extend the understanding of the effects of C. aerizusa extract on A549 lung carcinoma, examining its influence on various signaling pathways, malignancy, migration, and invasion.

Materials and methods: PCR was used to measure mRNA expression, targeting PTEN, Akt, mTOR, STAT-3, IL-6, VEGF, and HIF1α. Additionally, Western Blot analysis was adopted to assess PTEN, p-Akt, Akt, p-mTOR, and p-STAT-3 protein expressions. Wound healing and invasion assays were performed to measure the migration and invasion capabilities of A549 cells post-treatment with C. aerizusa extract.

Results: The mRNA expression analysis showed an increase in Akt and m-TOR but a decrease in PTEN and STAT-3 after 24 hours of treatment with C. aerizusa extract. At the protein level, there was a downregulation of p-Akt, Akt, p-mTOR, and p-STAT-3, while PTEN increased during 24-hour treatment. Wound healing and invasion assay results showed a weakened ability of A549 cells after a 24-hour treatment with C. aerizusa extract. Moreover, IL-6 and HIF-1α mRNA expression levels decreased during 24 hours, while VEGF mRNA had a slight decrease compared to untreated cells.

Conclusion: In conclusion, the ergosteroids present in marine sponge C. aerizusa extract signified a remarkable reduction in malignancy, migration, and invasion capabilities in A549 lung carcinoma cells. These results suggested their promising candidacy for future anti-angiogenesis in anticancer therapy.

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来源期刊
Journal of Experimental Pharmacology
Journal of Experimental Pharmacology Medicine-Pharmacology (medical)
CiteScore
7.40
自引率
0.00%
发文量
43
审稿时长
16 weeks
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