lnc-MEG3和miR-155联合替代可抑制多发性骨髓瘤的肿瘤。

IF 3 4区医学 Q2 GENETICS & HEREDITY

Epigenomics Pub Date : 2025-01-16 DOI:10.1080/17501911.2025.2453413

Nashwa El-Khazragy, Sara Elsayed Abdelrahman, Amal Darwish, Eman H A Hemida

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引用次数: 0

摘要

目的：与个体生物标志物替代相比，研究lncRNA MEG3和miR-155同时过表达（称为“双重打击”）对多发性骨髓瘤（MM）细胞的生物学影响。材料和方法：用meg3过表达的质粒和miR-155模拟物转染人MM细胞。在转染的细胞和临床样本中评估细胞毒性、细胞凋亡和基因表达。结果：MEG3和miR-155在MM患者中显著下调，低表达水平与疾病晚期和较差的生存率相关。双重过表达诱导了MM细胞的强毒性作用。结论：MEG3和miR-155是MM中潜在的肿瘤抑制因子。同时过表达这两种生物标志物可能代表一种新的治疗策略，它们的水平可以作为诊断和预后指标。

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Combined replacement of lnc-MEG3 and miR-155 elicit tumor suppression in multiple myeloma.

Aims: To investigate the biological impact of simultaneous overexpression of lncRNA MEG3 and miR-155, termed a "double hit," on multiple myeloma (MM) cells compared to individual biomarker substitution.

Materials and methods: Human MM cells were transfected with MEG3-overexpressed plasmids and miR-155 mimics. Cell cytotoxicity, apoptosis, and gene expression were evaluated in transfected cells and clinical samples.

Results: MEG3 and miR-155 were significantly downregulated in MM patients, with lower expression levels correlating with advanced disease stages and poorer survival. Dual overexpression induced potent cytotoxic effects in MM cells.

Conclusion: MEG3 and miR-155 are potential tumor suppressors in MM. Simultaneous overexpression of both biomarkers could represent a novel therapeutic strategy, and their levels could serve as diagnostic and prognostic markers.

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来源期刊

Epigenomics GENETICS & HEREDITY-

CiteScore

5.80

自引率

2.60%

发文量

审稿时长

>12 weeks

期刊介绍： Epigenomics provides the forum to address the rapidly progressing research developments in this ever-expanding field; to report on the major challenges ahead and critical advances that are propelling the science forward. The journal delivers this information in concise, at-a-glance article formats – invaluable to a time constrained community. Substantial developments in our current knowledge and understanding of genomics and epigenetics are constantly being made, yet this field is still in its infancy. Epigenomics provides a critical overview of the latest and most significant advances as they unfold and explores their potential application in the clinical setting.