{"title":"2014 - 2021年中国血液感染大肠埃希菌延伸谱β-内酰胺酶表型和基因型特征及时空变化","authors":"Sayyed Salman, Hao Xu, Yunbo Chen, Jinru Ji, Zhiying Liu, Yonghong Xiao","doi":"10.1186/s12941-025-00774-y","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To examine the characteristics and spatiotemporal changes in the phenotypes and genotypes of extended-spectrum β-lactamases (ESBLs) in Escherichia coli strains isolated from bloodstream infections (BSIs) across China between 2014 and 2021.</p><p><strong>Methods: </strong>983 ESBL-positive E. coli strains were collected from BSIs in 66 hospitals across different geographic regions in China from 2014 to 2021. The phenotypic confirmation of ESBL was performed through the double-disc diffusion method. The genetic type was determined using polymerase chain reaction (PCR) followed by DNA sequencing.</p><p><strong>Results: </strong>Between 2014 and 2021, the prevalence of ESBL-positive E. coli steadily decreased from 61.2 to 49.6%. Among 983 phenotypically confirmed ESBL-positive E. coli, 763 (77.6%) were confirmed to carry ESBL genes, with the majority being of the CTX-M type, which is further divided into 23 subtypes and dominated by the CTX-M-9 and CTX-M-1 groups, with 457/763 and 333/763, respectively. Other ESBLs and ampC genes, such as bla<sub>OXA-1</sub>, bla<sub>CMY</sub>, and bla<sub>DHA-1</sub>, often coexisted with either the CTX-M-9 or CTX-M-1 groups. bla<sub>CTX-M-14</sub> (34.3%, 157/457) and bla<sub>CTX-M-55</sub> (45.9%, 153/333) were the dominant subtypes in the CTX-M-9 and CTX-M-1 groups, respectively. A notable increase in bla<sub>CTX-M-27</sub> was observed, particularly from 2019 to 2021, with 26.4%, 23.1%, and 25.8% in all genotypes. Regarding the geographical distribution of the ESBLs, the highest rate of ESBL genetic positivity was observed in Southwest China, accounting for 84.9% (45/53), and the lowest was observed in Northeast China, with 73.2% (30/41). The abundance of the bla<sub>CTX-M-27</sub> genotype, in particular, exhibited a notable increase in Southwest China, with 31.4% (14/45) of the strains exhibiting this genotype, followed by the CTX-M-55 genotype, with 13.6% (6/45) of the strains exhibiting this genotype.</p><p><strong>Conclusions: </strong>This study demonstrated a steadily decreasing trend in the incidence of ESBLs and predominant CTX-M type ESBLs, particularly the CTX-M-9 and CTX-M-1 groups, in E. coli strains across China, a notable increase in the bla<sub>CTX-M-27</sub> genotype and regional variations in the ESBL gene distribution were detected.</p>","PeriodicalId":8052,"journal":{"name":"Annals of Clinical Microbiology and Antimicrobials","volume":"24 1","pages":"7"},"PeriodicalIF":4.6000,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11743024/pdf/","citationCount":"0","resultStr":"{\"title\":\"Characteristics and spatiotemporal changes in phenotypes and genotypes of extended-spectrum β-lactamases in Escherichia coli isolated from bloodstream infections in China from 2014 to 2021.\",\"authors\":\"Sayyed Salman, Hao Xu, Yunbo Chen, Jinru Ji, Zhiying Liu, Yonghong Xiao\",\"doi\":\"10.1186/s12941-025-00774-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To examine the characteristics and spatiotemporal changes in the phenotypes and genotypes of extended-spectrum β-lactamases (ESBLs) in Escherichia coli strains isolated from bloodstream infections (BSIs) across China between 2014 and 2021.</p><p><strong>Methods: </strong>983 ESBL-positive E. coli strains were collected from BSIs in 66 hospitals across different geographic regions in China from 2014 to 2021. The phenotypic confirmation of ESBL was performed through the double-disc diffusion method. The genetic type was determined using polymerase chain reaction (PCR) followed by DNA sequencing.</p><p><strong>Results: </strong>Between 2014 and 2021, the prevalence of ESBL-positive E. coli steadily decreased from 61.2 to 49.6%. Among 983 phenotypically confirmed ESBL-positive E. coli, 763 (77.6%) were confirmed to carry ESBL genes, with the majority being of the CTX-M type, which is further divided into 23 subtypes and dominated by the CTX-M-9 and CTX-M-1 groups, with 457/763 and 333/763, respectively. Other ESBLs and ampC genes, such as bla<sub>OXA-1</sub>, bla<sub>CMY</sub>, and bla<sub>DHA-1</sub>, often coexisted with either the CTX-M-9 or CTX-M-1 groups. bla<sub>CTX-M-14</sub> (34.3%, 157/457) and bla<sub>CTX-M-55</sub> (45.9%, 153/333) were the dominant subtypes in the CTX-M-9 and CTX-M-1 groups, respectively. A notable increase in bla<sub>CTX-M-27</sub> was observed, particularly from 2019 to 2021, with 26.4%, 23.1%, and 25.8% in all genotypes. Regarding the geographical distribution of the ESBLs, the highest rate of ESBL genetic positivity was observed in Southwest China, accounting for 84.9% (45/53), and the lowest was observed in Northeast China, with 73.2% (30/41). The abundance of the bla<sub>CTX-M-27</sub> genotype, in particular, exhibited a notable increase in Southwest China, with 31.4% (14/45) of the strains exhibiting this genotype, followed by the CTX-M-55 genotype, with 13.6% (6/45) of the strains exhibiting this genotype.</p><p><strong>Conclusions: </strong>This study demonstrated a steadily decreasing trend in the incidence of ESBLs and predominant CTX-M type ESBLs, particularly the CTX-M-9 and CTX-M-1 groups, in E. coli strains across China, a notable increase in the bla<sub>CTX-M-27</sub> genotype and regional variations in the ESBL gene distribution were detected.</p>\",\"PeriodicalId\":8052,\"journal\":{\"name\":\"Annals of Clinical Microbiology and Antimicrobials\",\"volume\":\"24 1\",\"pages\":\"7\"},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2025-01-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11743024/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Annals of Clinical Microbiology and Antimicrobials\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1186/s12941-025-00774-y\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annals of Clinical Microbiology and Antimicrobials","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s12941-025-00774-y","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Characteristics and spatiotemporal changes in phenotypes and genotypes of extended-spectrum β-lactamases in Escherichia coli isolated from bloodstream infections in China from 2014 to 2021.
Objective: To examine the characteristics and spatiotemporal changes in the phenotypes and genotypes of extended-spectrum β-lactamases (ESBLs) in Escherichia coli strains isolated from bloodstream infections (BSIs) across China between 2014 and 2021.
Methods: 983 ESBL-positive E. coli strains were collected from BSIs in 66 hospitals across different geographic regions in China from 2014 to 2021. The phenotypic confirmation of ESBL was performed through the double-disc diffusion method. The genetic type was determined using polymerase chain reaction (PCR) followed by DNA sequencing.
Results: Between 2014 and 2021, the prevalence of ESBL-positive E. coli steadily decreased from 61.2 to 49.6%. Among 983 phenotypically confirmed ESBL-positive E. coli, 763 (77.6%) were confirmed to carry ESBL genes, with the majority being of the CTX-M type, which is further divided into 23 subtypes and dominated by the CTX-M-9 and CTX-M-1 groups, with 457/763 and 333/763, respectively. Other ESBLs and ampC genes, such as blaOXA-1, blaCMY, and blaDHA-1, often coexisted with either the CTX-M-9 or CTX-M-1 groups. blaCTX-M-14 (34.3%, 157/457) and blaCTX-M-55 (45.9%, 153/333) were the dominant subtypes in the CTX-M-9 and CTX-M-1 groups, respectively. A notable increase in blaCTX-M-27 was observed, particularly from 2019 to 2021, with 26.4%, 23.1%, and 25.8% in all genotypes. Regarding the geographical distribution of the ESBLs, the highest rate of ESBL genetic positivity was observed in Southwest China, accounting for 84.9% (45/53), and the lowest was observed in Northeast China, with 73.2% (30/41). The abundance of the blaCTX-M-27 genotype, in particular, exhibited a notable increase in Southwest China, with 31.4% (14/45) of the strains exhibiting this genotype, followed by the CTX-M-55 genotype, with 13.6% (6/45) of the strains exhibiting this genotype.
Conclusions: This study demonstrated a steadily decreasing trend in the incidence of ESBLs and predominant CTX-M type ESBLs, particularly the CTX-M-9 and CTX-M-1 groups, in E. coli strains across China, a notable increase in the blaCTX-M-27 genotype and regional variations in the ESBL gene distribution were detected.
期刊介绍：
Annals of Clinical Microbiology and Antimicrobials considers good quality, novel and international research of more than regional relevance. Research must include epidemiological and/or clinical information about isolates, and the journal covers the clinical microbiology of bacteria, viruses and fungi, as well as antimicrobial treatment of infectious diseases.
Annals of Clinical Microbiology and Antimicrobials is an open access, peer-reviewed journal focusing on information concerning clinical microbiology, infectious diseases and antimicrobials. The management of infectious disease is dependent on correct diagnosis and appropriate antimicrobial treatment, and with this in mind, the journal aims to improve the communication between laboratory and clinical science in the field of clinical microbiology and antimicrobial treatment. Furthermore, the journal has no restrictions on space or access; this ensures that the journal can reach the widest possible audience.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
