微生物生产依托碱的研究进展

IF 3.7 2区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

ACS Synthetic Biology Pub Date : 2025-01-20 DOI:10.1021/acssynbio.4c00490

Ke Wang, Boya Cui, Yi Wang, Wei Luo

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引用次数: 0

摘要

外托碱是一种重要的天然次生代谢物，广泛应用于生物医学、新型化妆品开发和食品工业等领域。由于市场对ectoine的需求不断增加，人们正在探索更具成本效益的生产方法。随着合成生物学和代谢工程技术的快速发展，利用传统的嗜盐菌生产外托碱正逐渐被高产、环保的非嗜盐工程菌株所取代。通过在模型菌株中引入异托因合成途径，并通过各种代谢调控对发酵过程进行优化，可以实现异托因的高水平生产。本文从野生菌株筛选、突变选育、模式菌株代谢工程等方面综述了微生物生产外托碱的策略，旨在阐明目前的研究现状，为外托碱的工业化生产提供参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Microbial Production of Ectoine: A Review.

Ectoine is an important natural secondary metabolite widely used in biomedical fields, novel cosmetics development, and the food industry. Due to the increasing market demand for ectoine, more cost-effective production methods are being explored. With the rapid development of synthetic biology and metabolic engineering technologies, the production of ectoine using traditional halophilic bacteria is gradually being replaced by higher-yielding and environmentally friendly nonhalophilic engineered strains. By introducing the ectoine synthesis pathway into model strains and optimizing the fermentation process through various metabolic regulations, high-level production of ectoine can be achieved. This review focuses on strategies for the microbial production of ectoine, including screening of wild strains, mutation breeding, and metabolic engineering of model strains, to elucidate the current research status and provide insights for the industrial production of ectoine.

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来源期刊

ACS Synthetic Biology 生物-

CiteScore

8.00

自引率

10.60%

发文量

380

审稿时长

6-12 weeks

期刊介绍： The journal is particularly interested in studies on the design and synthesis of new genetic circuits and gene products; computational methods in the design of systems; and integrative applied approaches to understanding disease and metabolism. Topics may include, but are not limited to: Design and optimization of genetic systems Genetic circuit design and their principles for their organization into programs Computational methods to aid the design of genetic systems Experimental methods to quantify genetic parts, circuits, and metabolic fluxes Genetic parts libraries: their creation, analysis, and ontological representation Protein engineering including computational design Metabolic engineering and cellular manufacturing, including biomass conversion Natural product access, engineering, and production Creative and innovative applications of cellular programming Medical applications, tissue engineering, and the programming of therapeutic cells Minimal cell design and construction Genomics and genome replacement strategies Viral engineering Automated and robotic assembly platforms for synthetic biology DNA synthesis methodologies Metagenomics and synthetic metagenomic analysis Bioinformatics applied to gene discovery, chemoinformatics, and pathway construction Gene optimization Methods for genome-scale measurements of transcription and metabolomics Systems biology and methods to integrate multiple data sources in vitro and cell-free synthetic biology and molecular programming Nucleic acid engineering.