D. V. Prokopova, I. Yu. Eremchev, N. N. Losevsky, D. A. Belousov, S. K. Golubtsov, S. P. Kotova, A. V. Naumov
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Diffractive Optical Elements for Three-Dimensional Nanoscopy Using Rotating Light Fields
The authors study diffractive optical elements manufactured via contact printing on a dichromated gelatin and direct laser writing on photoresist. The elements modify the point-spread function for three-dimensional super resolution fluorescence microscopy. It is shown that both elements produce two-lobed rotating light fields that can be used for three-dimensional nanoscopy. Results from the subdiffractive localization of fluorescent labels are presented, and the accuracy of coordinate reconstruction is determined.
期刊介绍:
Bulletin of the Russian Academy of Sciences: Physics is an international peer reviewed journal published with the participation of the Russian Academy of Sciences. It presents full-text articles (regular, letters to the editor, reviews) with the most recent results in miscellaneous fields of physics and astronomy: nuclear physics, cosmic rays, condensed matter physics, plasma physics, optics and photonics, nanotechnologies, solar and astrophysics, physical applications in material sciences, life sciences, etc. Bulletin of the Russian Academy of Sciences: Physics focuses on the most relevant multidisciplinary topics in natural sciences, both fundamental and applied. Manuscripts can be submitted in Russian and English languages and are subject to peer review. Accepted articles are usually combined in thematic issues on certain topics according to the journal editorial policy. Authors featured in the journal represent renowned scientific laboratories and institutes from different countries, including large international collaborations. There are globally recognized researchers among the authors: Nobel laureates and recipients of other awards, and members of national academies of sciences and international scientific societies.