L. A. Wasserman, E. S. Gavrilina, L. V. Yurina, A. D. Vasilyeva, M. A. Rosenfeld
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Study of Thermal Denaturation of a Plasminogen Molecule Under Induced Oxidation
This article studies the thermal denaturation of a plasminogen molecule during induced oxidation by hypochlorite in a range of concentrations (30, 62.5, 125, and 250 µM). Using differential scanning calorimetry, it is determined that in the presence of an oxidizing agent, the enthalpy of denaturation of the plasminogen molecule decreases. This is most noticeable for the peak showing the melting of the K4–K5 kringle domains. These results are consistent with the previously obtained data on the oxidative modification of amino acid residues of plasminogen treated with different concentrations of hypochlorite using the HPLC-MS/MS method. Taken together, these data and the results of previous studies indicate that the structure of full-length plasminogen is adapted to moderate HOCl-induced oxidation.
期刊介绍：
Russian Journal of Physical Chemistry B: Focus on Physics is a journal that publishes studies in the following areas: elementary physical and chemical processes; structure of chemical compounds, reactivity, effect of external field and environment on chemical transformations; molecular dynamics and molecular organization; dynamics and kinetics of photoand radiation-induced processes; mechanism of chemical reactions in gas and condensed phases and at interfaces; chain and thermal processes of ignition, combustion and detonation in gases, two-phase and condensed systems; shock waves; new physical methods of examining chemical reactions; and biological processes in chemical physics.
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