肼基近红外荧光探针实时监测ONOO -脑缺血-再灌注损伤小鼠模型

IF 10.7 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Redox Biology Pub Date : 2025-01-13 DOI:10.1016/j.redox.2025.103494

Jinting Shang , Yan Yang , Yaojian Sun , Wanxia Gao , Kang Ma , Chen Wang , Xin Yu , Liping Li , Jiang Zheng , Na Zhao , XiJi Shu , Yibin Zhang

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引用次数: 0

摘要

脑缺血再灌注损伤（CIRI）模型中内源性过氧亚硝酸盐（ONOO−）的精确和选择性可视化技术对于理解其复杂的病理过程至关重要。本文介绍了一种用于快速、灵敏地检测ONOO−的长波荧光探针TJO，其检测限低至91 nM。此外，TJO表现出出色的荧光成像能力，使ONOO毒血症小鼠模型的详细可视化。这突出了它在实时监测ONOO毒血症相关病理情况方面的潜力。

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Real-time monitoring of ONOO⁻ in cerebral ischemia-reperfusion injury mouse models using a hydrazine-based NIR fluorescent probe

Accurate and selective techniques for visualizing endogenous peroxynitrite (ONOO⁻) in cerebral ischemia-reperfusion injury (CIRI) models are essential for understanding its complex pathological processes. Here, we introduced a longwave fluorescent probe TJO for detecting ONOO⁻ rapidly and sensitively, with a low detection limit of 91 nM. Furthermore, TJO exhibits excellent fluorescence imaging capabilities, enabling detailed visualization of ONOO⁻ in CIRI mice model. This highlights its potential for real-time monitoring of ONOO⁻-related pathological conditions.

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来源期刊

Redox Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

19.90

自引率

3.50%

发文量

318

审稿时长

25 days

期刊介绍： Redox Biology is the official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe. It is also affiliated with the International Society for Free Radical Research (SFRRI). This journal serves as a platform for publishing pioneering research, innovative methods, and comprehensive review articles in the field of redox biology, encompassing both health and disease. Redox Biology welcomes various forms of contributions, including research articles (short or full communications), methods, mini-reviews, and commentaries. Through its diverse range of published content, Redox Biology aims to foster advancements and insights in the understanding of redox biology and its implications.