核糖体分析揭示x射线照射后HEK293T细胞的动态翻译景观。

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2025-01-02 DOI:10.1016/j.ygeno.2025.110987

Jingyu Hou , Lei Yu , Canlan Wu , Saisai Wei , Xiangwei Gao

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引用次数: 0

摘要

x射线照射引起基因表达的广泛变化。位于中心法则的底部，翻译调控对环境刺激反应迅速，微调蛋白质水平。然而，x射线照射后mRNA翻译的整体观点仍不清楚。在这项研究中，我们使用核糖体分析系统地研究了x射线诱导的翻译交替。我们的研究揭示了x射线治疗后HEK293T细胞的暂时翻译抑制。mrna的一个子集通过绕过上游开放阅读框（uorf）经历了翻译上调。上调基因在MAPK信号通路中富集，如MAPKBP1。抑制MAPKBP1抑制x射线诱导的细胞凋亡。此外，我们发现x射线处理诱导长链非编码rna （lncRNAs）中由小开放阅读框（smorf）编码的新肽。总的来说，我们的研究结果提供了真核细胞在x射线治疗后的翻译景观的全面概述，为DNA损伤反应提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Ribosome profiling reveals dynamic translational landscape following X-ray irradiation

X-ray irradiation induces widespread changes in gene expression. Positioned at the bottom of the central dogma, translational regulation responds swiftly to environmental stimuli, fine-tuning protein levels. However, the global view of mRNA translation following X-ray exposure remains unclear. In this study, we systematically investigated X-ray-induced translational alternation using ribosome profiling. Our study revealed a temporary translation inhibition in HEK293T cells following X-ray treatment. A subset of mRNAs experienced translational upregulation by bypassing upstream open reading frames (uORFs). The upregulated genes were enriched in the MAPK signaling pathway, such as MAPKBP1. Suppression of MAPKBP1 inhibited X-ray-induced cell apoptosis. Furthermore, we identified the induction of novel peptides encoded by small open reading frames (smORFs) within long non-coding RNAs (lncRNAs) upon X-ray treatment. Overall, our findings provide a comprehensive overview of the translational landscape within eukaryotic cells following X-ray treatment, offering new insights into DNA damage response.

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.