卡林：用于同时读取谱系历史和基因表达的小鼠系。

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4310-5_14

Sarah Bowling, Fernando D Camargo

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引用次数: 0

摘要

crispr激活修复谱系追踪（CARLIN）小鼠系使用DNA条形码实现体内细胞谱系的高分辨率追踪（Bowling等，cell 181, 1410-1422）。e27, 2020)。CARLIN小鼠含有表达的条形码，允许同时询问来自单个细胞的谱系和基因表达信息。此外，条形码编辑是完全可诱导的，因此可以在发育或成年的任何时间点进行细胞谱系标记。本章详细介绍了CARLIN小鼠的维持、诱导条形码以及从DNA、RNA和单细胞RNA测序文库中扩增CARLIN条形码用于下一代测序所遵循的协议。

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CARLIN: A Mouse Line for Simultaneous Readout of Lineage Histories and Gene Expression.

The CRISPR-activated repair lineage tracing (CARLIN) mouse line uses DNA barcoding to enable high-resolution tracing of cell lineages in vivo (Bowling et al, Cell 181, 1410-1422.e27, 2020). CARLIN mice contain expressed barcodes that allow simultaneous interrogation of lineage and gene expression information from single cells. Furthermore, barcode editing is fully inducible, resulting in cell lineage labeling that can be performed at any time point in development or adulthood. This chapter details the protocols followed for maintaining CARLIN mice, inducing barcoding, and amplifying the CARLIN barcode from DNA, RNA, and single-cell RNA-sequencing libraries for next-generation sequencing.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.