HOX基因在血栓细胞发育中的作用研究。

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4322-8_8

Jabila Mary, Pudur Jagadeeswaran

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引用次数: 0

摘要

在实验室中，我们以斑马鱼为模型生物研究血小板生成和止血，以揭示血小板分化和发育的机制。我们已经在我们早期的工作中表明，血小板的功能等同，并具有类似于巨核细胞的转录机制。我们最近发现了hox基因在它们的发育中起作用的证据。我们使用了基因敲除和血小板定量分析来了解这些古老的发育调节因子对血小板生成的影响。在本章中，我们描述了发现这些hox基因的方法。

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Studying the Role of HOX Genes in Thrombocyte Development.

In our laboratory, we study thrombopoiesis and hemostasis using zebrafish as a model organism to unravel the mechanisms of differentiation and development of thrombocytes. We have shown in our earlier work that thrombocytes are functional equivalents of platelets and have transcriptional machinery similar to megakaryocytes. We recently found evidence that hox genes play a role in their development. We used piggyback gene knockdown and thrombocyte quantification assays to understand the influence of these ancient developmental regulators on thrombopoiesis. In this chapter, we describe methods used to discover these hox genes.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.