空间基因组方法研究小鼠脑组织中HOX基因。

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4322-8_16

Ashish Shelar, Anasuya Dighe

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引用次数: 0

摘要

空间转录组学工具是研究组织内靶向基因表达模式的一种即将到来的强大方法。这些工具在保持组织完整性的同时提供了可视化和理解基因表达的独特优势，从而维持了基因的空间背景。Curio是一个强大的空间转录组学工具，可以高效地促进整个转录组的高通量综合空间基因表达分析。在这里，我们提出了使用Curio进行小鼠脑组织全转录组基因表达分析的生物信息学方案。具体来说，我们演示了使用计算技术来可视化各种HOX基因在小鼠大脑中的表达模式。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Spatial Genomic Approaches to Investigate HOX Genes in Mouse Brain Tissues.

Spatial transcriptomic tools are an upcoming and powerful way to investigate targeted gene expression patterns within tissues. These tools offer the unique advantage of visualizing and understanding gene expression while preserving tissue integrity, thereby maintaining the spatial context of genes. Curio is a robust spatial transcriptomic tool that facilitates high throughput comprehensive spatial gene expression analysis across the entir e transcriptome with high efficiency. Here, we present a bioinformatics protocol for performing whole transcriptome gene expression analysis of mouse brain tissue using Curio. Specifically, we demonstrate using computational techniques to visualize expression patterns of various HOX genes in the mouse brain.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.