分析ChIP-Seq数据的指南：通过QC和分析考虑的过程。

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4322-8_14

Bony De Kumar, Jaya Krishnan

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引用次数: 0

摘要

ChIP-Seq用于研究dna -蛋白质相互作用，揭示染色质状态和转录因子的基因调控特性。ChIP-Seq涉及免疫沉淀，然后使用下一代测序方法进行测序。ENCODE联盟为ChIP-Seq分析提供了广泛的指导方针。同时，适当的QC指标和解释结果的知识对于一个好的ChIP-Seq实验是必不可少的。本章概述了用于ChIP-Seq分析的各种QC指标和分析工具，以便更好地理解结果。

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Guidelines to Analyze ChIP-Seq Data: Journey Through QC and Analysis Considerations.

ChIP-Seq is used to study DNA-protein interactions, unraveling chromatin states and gene regulatory properties of transcription factors. ChIP-Seq involves immunoprecipitation followed by sequencing using Next-Generation sequencing approaches. The ENCODE consortium provides extensive guidelines for ChIP-Seq analysis. Meanwhile, appropriate QC metrics and knowledge to interpret outcomes are essential for a good ChIP-Seq experiment. This chapter outlines the various QC metrics and analytical tools for ChIP-Seq analysis to provide a better understanding of the results.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.