{"title":"[基于ATR-FTIR方法原理的I-dOne测量装置的细菌鉴定性能评价]。","authors":"Reo Sasaki, Yumiko Funashima, Zenzo Nagasawa, Kentaro Wakamatsu","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Recently, mass spectrometers based on MALDI-TOF MS, which can rapidly identify microorganisms, have become widely used, contributing to the proper use of antimicrobial agents and diagnosis of infectious diseases through rapid reporting of bacterial species. We had the opportunity to evaluate the performance of the I-dOne, a microorganism identification system using the ATR-FTIR method. A total of 174 strains, 78 of 11 Grampositive bacteria and 96 of 12 Gram-negative bacteria, registered in the I-dOne database were used. The strains were identified using the mass spectrometer MALDI Biotyper (Bruker Japan Inc.) and compared with the I-dOne identification results. The identification agreement rate was 73.3% (255 out of 348 shares), including Gram-positive bacteria and Gram-negative bacteria. I-dOne is expected to improve its identification accuracy through software updates and database additions and improvements.</p>","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"34 1","pages":"17-22"},"PeriodicalIF":0.0000,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Evaluation of Bacterial Identification Performance Using the I-dOne Measuring Device Based on the Principle of the ATR-FTIR Method].\",\"authors\":\"Reo Sasaki, Yumiko Funashima, Zenzo Nagasawa, Kentaro Wakamatsu\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Recently, mass spectrometers based on MALDI-TOF MS, which can rapidly identify microorganisms, have become widely used, contributing to the proper use of antimicrobial agents and diagnosis of infectious diseases through rapid reporting of bacterial species. We had the opportunity to evaluate the performance of the I-dOne, a microorganism identification system using the ATR-FTIR method. A total of 174 strains, 78 of 11 Grampositive bacteria and 96 of 12 Gram-negative bacteria, registered in the I-dOne database were used. The strains were identified using the mass spectrometer MALDI Biotyper (Bruker Japan Inc.) and compared with the I-dOne identification results. The identification agreement rate was 73.3% (255 out of 348 shares), including Gram-positive bacteria and Gram-negative bacteria. I-dOne is expected to improve its identification accuracy through software updates and database additions and improvements.</p>\",\"PeriodicalId\":74740,\"journal\":{\"name\":\"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology\",\"volume\":\"34 1\",\"pages\":\"17-22\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-12-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
近年来,基于MALDI-TOF MS的质谱仪因其能快速识别微生物而得到广泛应用,有助于抗菌药物的正确使用和通过快速报告细菌种类来诊断传染病。我们有机会评估I-dOne的性能,这是一种使用ATR-FTIR方法的微生物鉴定系统。共使用I-dOne数据库中登记的174株,其中11株革兰氏阳性菌78株,12株革兰氏阴性菌96株。采用MALDI Biotyper (Bruker Japan Inc.)质谱仪对菌株进行鉴定,并与I-dOne鉴定结果进行比较。包括革兰氏阳性菌和革兰氏阴性菌在内的鉴定符合率为73.3%(348份中255份)。预计I-dOne将通过软件更新和数据库添加和改进来提高其识别准确性。
[Evaluation of Bacterial Identification Performance Using the I-dOne Measuring Device Based on the Principle of the ATR-FTIR Method].
Recently, mass spectrometers based on MALDI-TOF MS, which can rapidly identify microorganisms, have become widely used, contributing to the proper use of antimicrobial agents and diagnosis of infectious diseases through rapid reporting of bacterial species. We had the opportunity to evaluate the performance of the I-dOne, a microorganism identification system using the ATR-FTIR method. A total of 174 strains, 78 of 11 Grampositive bacteria and 96 of 12 Gram-negative bacteria, registered in the I-dOne database were used. The strains were identified using the mass spectrometer MALDI Biotyper (Bruker Japan Inc.) and compared with the I-dOne identification results. The identification agreement rate was 73.3% (255 out of 348 shares), including Gram-positive bacteria and Gram-negative bacteria. I-dOne is expected to improve its identification accuracy through software updates and database additions and improvements.