通过纳米技术和精油处理革兰氏阳性和革兰氏阴性细菌对左氧氟沙星的生物膜耐药性

IF 2.7 4区 医学 Q2 PHARMACOLOGY & PHARMACY
Sarhan Omnia Mohamed
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引用次数: 0

摘要

细菌产生生物膜已成为抗生素耐药性上升的一个主要因素。脂质纳米胶囊(lnc)由于其独特的性质和携带多种治疗性化合物的能力,最近成为一种创新的药物输送平台。目的建立、优化并评价含左氧氟沙星薄荷油乳剂(o/w)对耐药菌形成生物膜的抗菌效果。方法采用精油,特别是具有抗真菌特性的薄荷油,代替传统的中链甘油三酯制备脂质纳米载体,利用交替的表面活性剂类型(solutool HS 15和Cremophor EL)和不同的油与表面活性剂的比例(2:1和1:1)。考虑到lfx - lnc配方的物理性质,包括粒径、zeta电位、透射电子显微镜和多分散性指数,研究人员选择了油与表面活性剂比例为2:1的lfx - lnc配方进行进一步研究。对LFX-LNCs的抗菌效率进行了评估,揭示了它们能够根除革兰氏阴性病原体的生物膜,包括大肠杆菌(E. coli)和铜绿假单胞菌(P. aeruginosa),以及革兰氏阳性菌株,如金黄色葡萄球菌(S. aureus)。结果LFX-LNCs的平均粒径范围为30.86±0.54 nm ~ 68.36±0.56 nm,粒径分布较窄,zeta电位为负(-1.56±0.24 ~ -20.2±2.15 mV), PDI为0.062±0.006 ~ 0.26±0.002。通过透射电子显微镜(TEM)分析,脂质纳米胶囊通常在纳米尺寸范围内呈现球形形态。抗菌活性评估显示,EL 2:1具有最显著的抗菌效果,其特点是粒径减小,抑制区高达(2.43±0.24 cm),对铜绿假单胞菌、金黄色葡萄球菌和大肠杆菌等几种病原菌具有良好的抑菌效果。结论LFX-LNCs对生物膜细菌感染的未愈合创面有较好的治疗效果。图形抽象
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Tackling Biofilm Resistance of Gram-Positive and Gram-Negative Bacteria Against Levofloxacin via Nanotechnology and Essential Oils

Introduction

The generation of biofilms by bacteria has become a major factor in the rise of antibiotic resistance. Lipid nano-capsules (LNCs) have recently emerged as an innovative platform for drug delivery, due to their unique properties and ability to carry a wide array of therapeutic chemical compounds.

Objectives

The objective of this research was to create, optimize, and evaluate the antibiofilm efficacy of a peppermint oil emulsion (o/w) containing levofloxacin against resistant bacteria via biofilm formation.

Methods

Essential oils, particularly peppermint oil known for its antifungal properties, were employed instead of traditional medium chain triglycerides to formulate lipid nanocarriers, utilizing alternating surfactant types (Solutol HS 15 and Cremophor EL) and differing oil to surfactant ratios (2:1 and 1:1). The LFX-LNCs formula, with a 2:1 oil to surfactant ratio, was selected for further investigation due to its physical properties, including particle size, zeta potential, transmission electron microscopy, and polydispersity index. The antibacterial efficiency of LFX-LNCs was evaluated, revealing their ability to eradicate established biofilms of Gram-negative pathogens, including Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa), as well as Gram-positive strains such as Staphylococcus aureus (S. aureus).

Results

The mean particle size of LFX-LNCs varied from 30.86 ± 0.54 nm to 68.36 ± 0.56 nm, demonstrating a narrow size distribution, a negative zeta potential (-1.56 ± 0.24 to -20.2 ± 2.15 mV), and a polydispersity index (PDI) ranging from 0.062 ± 0.006 to 0.26 ± 0.002. Lipid nanocapsules generally exhibit a spherical morphology within the nanometric size range when analyzed by transmission electron microscopy (TEM). The antimicrobial activity assessment revealed that EL 2:1 exhibited the most significant antimicrobial efficacy, characterized by a reduced particle size and an inhibition zone measuring up to (2.43 ± 0.24 cm), demonstrating promising results against several pathogenic strains, including P. aeruginosa, S. aureus, and E. coli.

Conclusion

This study illustrates the efficacy of LFX-LNCs in the treatment of non-healing wounds infected with biofilm-forming bacteria.

Graphical Abstract

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来源期刊
Journal of Pharmaceutical Innovation
Journal of Pharmaceutical Innovation PHARMACOLOGY & PHARMACY-
CiteScore
3.70
自引率
3.80%
发文量
90
审稿时长
>12 weeks
期刊介绍: The Journal of Pharmaceutical Innovation (JPI), is an international, multidisciplinary peer-reviewed scientific journal dedicated to publishing high quality papers emphasizing innovative research and applied technologies within the pharmaceutical and biotechnology industries. JPI''s goal is to be the premier communication vehicle for the critical body of knowledge that is needed for scientific evolution and technical innovation, from R&D to market. Topics will fall under the following categories: Materials science, Product design, Process design, optimization, automation and control, Facilities; Information management, Regulatory policy and strategy, Supply chain developments , Education and professional development, Journal of Pharmaceutical Innovation publishes four issues a year.
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