流式细胞术免疫表型分析在成熟b细胞淋巴瘤/白血病诊断中的应用。

IF 2.7 2区医学 Q2 PATHOLOGY

Human pathology Pub Date : 2025-02-01 DOI:10.1016/j.humpath.2024.105711

Hong Fang, Sa A. Wang, L. Jeffrey Medeiros, Wei Wang

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引用次数: 0

摘要

流式细胞术免疫表型分析是成熟b细胞淋巴瘤/白血病诊断中不可缺少的重要工具，特别是对于越来越流行的小细针穿刺和针芯活检标本的诊断。流式细胞术免疫分型（FCI）有几个优点。由于其多参数特性，FCI可以定性和定量地分析多个抗原同时在同一目标细胞上的表达。在诊断过程中，FCI可以在其他辅助研究（如免疫组织化学和分子研究）的形态学评估和分诊之前提供时间敏感和有价值的信息。在这篇综述中，我们旨在为FCI在成熟b细胞肿瘤的诊断检查中提供通用和实用的方法。本文还讨论了常见成熟b细胞肿瘤的免疫表型特征以及与FCI相关的诊断挑战和陷阱。

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Application of flow cytometry immunophenotypic analysis for the diagnosis of mature B-cell lymphomas/leukemias

Flow cytometry immunophenotyping (FCI) is an important and indispensable tool in the diagnosis of mature B-cell lymphomas/leukemias, particularly for small fine needle aspiration and needle core biopsy specimens which are becoming increasingly popular for diagnostic purposes. FCI has several advantages. Given its multiparametric nature, FCI can analyze the expression of multiple antigens simultaneously on the same cell of interest, qualitatively and quantitively. During the diagnostic process, FCI can provide time sensitive and valuable information for triage of other ancillary studies such as immunohistochemical and molecular studies. In this review, we aim to provide common and practical approaches for using FCI in the diagnostic workup of mature B-cell neoplasms. The immunophenotypic features of common mature B-cell neoplasms as well as diagnostic challenges and pitfalls associated with FCI are also discussed.

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来源期刊

Human pathology 医学-病理学

CiteScore

5.30

自引率

6.10%

发文量

206

审稿时长

21 days

期刊介绍： Human Pathology is designed to bring information of clinicopathologic significance to human disease to the laboratory and clinical physician. It presents information drawn from morphologic and clinical laboratory studies with direct relevance to the understanding of human diseases. Papers published concern morphologic and clinicopathologic observations, reviews of diseases, analyses of problems in pathology, significant collections of case material and advances in concepts or techniques of value in the analysis and diagnosis of disease. Theoretical and experimental pathology and molecular biology pertinent to human disease are included. This critical journal is well illustrated with exceptional reproductions of photomicrographs and microscopic anatomy.