血友病关节滑膜组织的金属蛋白组学分析：金属超载和发病机制的见解。

IF 5.5 2区医学 Q1 HEMATOLOGY

Journal of Thrombosis and Haemostasis Pub Date : 2025-03-01 DOI:10.1016/j.jtha.2024.12.013

Dasheng Luo , Liujie Zheng , Mingyang Ding , Defu Yu , Tao Chen , Ke Zheng , Juehua Jing , Yunfeng Yao

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引用次数: 0

摘要

背景：血友病关节病（HA）是一种以局部铁超载为特征的关节疾病，起源于红细胞破裂，与滑膜病变密切相关。然而，临床HA滑膜样本的精确分子特征仍有待确定。目的：为了深入了解透明质酸滑膜组织病变，我们利用金属蛋白策略比较了透明质酸与骨关节炎（OA）和类风湿性关节炎（RA）的金属和蛋白质光谱。患者/方法：我们收集HA， OA和RA患者的滑膜样本。通过金属组学和蛋白质组学分别获得组织金属和蛋白质谱。最后，金属蛋白质组学策略比较金属含量、蛋白质、金属蛋白和所涉及的生命过程。结果和结论：我们的金属组学分析显示HA滑膜样品中重金属含量明显增加，特别是砷（As）和汞（Hg）。通过蛋白质组学，我们描述了特定的金属蛋白，并确定了金属与途径之间的相关性。这些发现为HA的发病机制提供了有价值的见解，并为以铁超载为特征的疾病提供了潜在的治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Metalloproteomic analysis of hemophilic arthropathy synovial tissue: insights into metal overload and pathogenesis

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Metalloproteomic analysis of hemophilic arthropathy synovial tissue: insights into metal overload and pathogenesis

Background

Hemophilic arthropathy (HA) is a joint disease characterized by local iron overload stemming from erythrocyte rupture and closely linked to synovial lesions. However, the precise molecular characteristics of clinical HA synovial samples remain to be defined.

Objectives

To gain insight into HA synovial tissue lesions, we utilized a metalloprotein strategy to compare the metal and protein spectra of HA with those of osteoarthritis and rheumatoid arthritis.

Methods

We collected synovial samples from patients with HA, osteoarthritis, and rheumatoid arthritis. Tissue metal and protein profiles were obtained by metallomics and proteomics, respectively. Finally, metalloproteomics strategies compared metal content, proteins, metalloproteins, and the life processes involved.

Results

Our metallomics analysis revealed an explicit increase in heavy metal content, particularly arsenic and mercury, in HA synovial samples. Through proteomics, we delineated specific metalloproteins and identified correlations between metals and pathways.

Conclusion

These findings yield valuable insights into the pathogenesis of HA and offer potential therapeutic targets for conditions characterized by iron overload.

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来源期刊

Journal of Thrombosis and Haemostasis 医学-外周血管病

CiteScore

24.30

自引率

3.80%

发文量

321

审稿时长

1 months

期刊介绍： The Journal of Thrombosis and Haemostasis (JTH) serves as the official journal of the International Society on Thrombosis and Haemostasis. It is dedicated to advancing science related to thrombosis, bleeding disorders, and vascular biology through the dissemination and exchange of information and ideas within the global research community. Types of Publications: The journal publishes a variety of content, including: Original research reports State-of-the-art reviews Brief reports Case reports Invited commentaries on publications in the Journal Forum articles Correspondence Announcements Scope of Contributions: Editors invite contributions from both fundamental and clinical domains. These include: Basic manuscripts on blood coagulation and fibrinolysis Studies on proteins and reactions related to thrombosis and haemostasis Research on blood platelets and their interactions with other biological systems, such as the vessel wall, blood cells, and invading organisms Clinical manuscripts covering various topics including venous thrombosis, arterial disease, hemophilia, bleeding disorders, and platelet diseases Clinical manuscripts may encompass etiology, diagnostics, prognosis, prevention, and treatment strategies.