IF 14.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Chenxi Feng, Qiongxiang Yan, Xianyi Li, Hong Zhao, Hua Huang, Xinshuai Zhang
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引用次数: 0

摘要

麦角硫因是人类从膳食中摄取的一种微量营养素。然而,人类肠道细菌中参与麦角硫因分解代谢的酶尚未被确定。在本文中,我们描述了肠道细菌分解这种微量营养素的硫化途径,其中涉及黄嘌呤氧化还原酶家族(XOR)成员催化的前所未有的还原脱硫反应,XOR是一类含钼黄蛋白。值得注意的是,这是已知的第一个由 XOR 催化的 C-S 键裂解反应。利用从人类肠道细菌 Blautia producta ATCC 27340 中提取的异源酶进行体外重建,获得了这一途径运行的证据。这种分解活动使 B. producta ATCC 27340 能够使用麦角硫因作为替代电子受体源。研究表明,该途径酶的同源物不仅存在于人类肠道细菌中,还存在于许多环境细菌中,这表明这种分解策略分布广泛。关于含硫代谢物,这一发现为了解人类肠道以外各种缺氧栖息地的生物地球化学硫循环提供了重要信息,而且还为设计控制肠道硫化氢(H2S)产生的新方法提供了重要信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Discovery of a Gut Bacterial Pathway for Ergothioneine Catabolism

Discovery of a Gut Bacterial Pathway for Ergothioneine Catabolism
Ergothioneine is a diet-derived micronutrient for humans. However, enzymes involved in the catabolism of ergothioneine in human gut bacteria have not yet been identified. Herein, we characterize a sulfidogenic pathway for gut bacterial catabolism of this micronutrient, which involves an unprecedented reductive desulfurization reaction catalyzed by members of the xanthine oxidoreductase family (XOR), a class of molybdenum-containing flavoproteins. Notably, this is the first C–S bond cleavage reaction known to be catalyzed by XORs. Evidence for operation of this pathway was gained through in vitro reconstruction using heterologously produced enzymes derived from the human gut bacterium Blautia producta ATCC 27340. This catabolic activity enables B. producta ATCC 27340 to use ergothioneine as an alternative electron acceptor source. Homologues of the pathway enzymes are shown to be present not only in human gut bacteria but also in many environmental bacteria, suggesting the wide distribution of this catabolic strategy. In relation to the sulfur-containing metabolite, this discovery provides significant insight into biogeochemical sulfur cycling in diverse anoxic habitats beyond the human gut and, moreover, the design of new approaches for controlling intestinal hydrogen sulfide (H2S) production.
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来源期刊
CiteScore
24.40
自引率
6.00%
发文量
2398
审稿时长
1.6 months
期刊介绍: The flagship journal of the American Chemical Society, known as the Journal of the American Chemical Society (JACS), has been a prestigious publication since its establishment in 1879. It holds a preeminent position in the field of chemistry and related interdisciplinary sciences. JACS is committed to disseminating cutting-edge research papers, covering a wide range of topics, and encompasses approximately 19,000 pages of Articles, Communications, and Perspectives annually. With a weekly publication frequency, JACS plays a vital role in advancing the field of chemistry by providing essential research.
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