冬眠灰熊心肌肌球蛋白超松弛增加作为一种节能机制。

IF 7 2区医学 Q1 ENDOCRINOLOGY & METABOLISM

Molecular Metabolism Pub Date : 2025-02-01 DOI:10.1016/j.molmet.2024.102084

Robbert J. Van der Pijl , Weikang Ma , Christopher T.A. Lewis , Line Haar , Amalie Buhl , Gerrie P. Farman , Marcus Rhodehamel , Vivek P. Jani , O Lynne Nelson , Chengxin Zhang , Henk Granzier , Julien Ochala

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引用次数: 0

摘要

目的：本研究的目的是确定心肌肌凝蛋白是否有助于冬眠哺乳动物心脏的能量保存。方法：分别从活动灰熊和冬眠灰熊的左心室分离心脏薄条；并进行负载Mant-ATP追踪分析，x射线衍射和蛋白质组学。主要发现：冬眠的灰熊表现出异常高比例的保存atp的超松弛心肌肌球蛋白分子，这可能是由于磷酸化水平和棒区稳定性的改变。结论：心肌肌球蛋白通过调节心脏功能抑制冬眠时心脏的能量需求。

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Increased cardiac myosin super-relaxation as an energy saving mechanism in hibernating grizzly bears

Aim

The aim of the present study was to define whether cardiac myosin contributes to energy conservation in the heart of hibernating mammals.

Methods

Thin cardiac strips were isolated from the left ventricles of active and hibernating grizzly bears; and subjected to loaded Mant-ATP chase assays, X-ray diffraction and proteomics.

Main findings

Hibernating grizzly bears displayed an unusually high proportion of ATP-conserving super-relaxed cardiac myosin molecules that are likely due to altered levels of phosphorylation and rod region stability.

Conclusions

Cardiac myosin depresses the heart's energetic demand during hibernation by modulating its function.

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来源期刊

Molecular Metabolism ENDOCRINOLOGY & METABOLISM-

CiteScore

14.50

自引率

2.50%

发文量

219

审稿时长

43 days

期刊介绍： Molecular Metabolism is a leading journal dedicated to sharing groundbreaking discoveries in the field of energy homeostasis and the underlying factors of metabolic disorders. These disorders include obesity, diabetes, cardiovascular disease, and cancer. Our journal focuses on publishing research driven by hypotheses and conducted to the highest standards, aiming to provide a mechanistic understanding of energy homeostasis-related behavior, physiology, and dysfunction. We promote interdisciplinary science, covering a broad range of approaches from molecules to humans throughout the lifespan. Our goal is to contribute to transformative research in metabolism, which has the potential to revolutionize the field. By enabling progress in the prognosis, prevention, and ultimately the cure of metabolic disorders and their long-term complications, our journal seeks to better the future of health and well-being.